cADDis – A New Handle on Cilia Signaling

The Proceedings of the National Academy of Sciences published another new scientific breakthrough made possible with our cADDis fluorescent biosensors. Our sensors indicate changes in cell signals with changes in fluorescence intensity and provide a window into the hidden machinery of the living cell.
 
In collaboration with Montana Molecular, researchers at the Geisinger Medical Center used cADDis to study cAMP regulation in primary cilia, a specialized compartment of cells. Understanding how levels of cAMP are balanced within the cell is critical to understanding disease as well as normal cell function.
 
By targeting Montana Molecular’s cADDis sensor to the cilia, our collaborators showed precisely how ciliary cAMP levels are controlled and how this process surprisingly differs from cAMP signaling in the rest of the cell.  
 

Moore, B.S., Stepanchick, A.N., Tewson, P.H., Hartle, C.M., Zhang, J., Quinn, A.M., Hughes, T.E., Mirshahi, T. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics. PNAS 2016

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