Introducing cADDis:
The simplest assay in the world for detecting cAMP

Measure cAMP signaling in real time, in the living cells of your choice.

Save time and money by switching to cADDis, a simple and robust assay for Gs and Gi signaling, without the need for co-factors, cell lysis, or complex liquid handling. cADDis is genetically-encoded and packaged in BacMam for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and plate readers.

Gs detection: Kinetics. Dose responses. Bright and reliable signals.

Measure kinetics and dose responses of Gs-mediated cAMP production with large and consistent fluorescence changes. cADDis is extremely bright with remarkable signal to noise (Z’ > 0.95).
Below: cADDis in live HEK293 cells stimulated with isoproterenol, a selective agonist of β-adrenergic receptors.

Gi detection:

cADDis detects Gi-mediated decreases in cAMP production, in real time, Gi assay yields Z’ values > 0.85. 
Below: cADDis in live HEK293 cells stimulated with quinpirole, a selective agonist of dopaminergic D2 receptors.

Order a complete cADDis Assay Kit. Visit our product page for more information about the revolutionary cADDis assay for cAMP. Custom packaging in lentivirus and AAV available upon request.