Montana Molecular is pleased to announce the publication of our live cell GPCR assays in the August 2013 edition of the Journal of Biomolecular Screening. A Multiplexed Fluorescent Assay for Independent Second-Messenger Systems: Decoding GPCR Activation in Living Cells J BIOMOL SCREEN August 2013 18: 797-806
The need in drug discovery and basic research to measure multiple second-messenger components of cell signaling pathways is increasing as the information about GPCR signaling indicates that pathway selectivity is more complex than originally imagined. When G-protein–coupled receptors activate the heterotrimeric protein, Gq, phospholipase C (PLC) is activated in turn. PLC cleaves phosphatidylinositol 4,5-bisphosphate (PIP2) to produce two second messengers: diacylglycerol (DAG), which remains in the plasma membrane, and inositol triphosphate (IP3), which diffuses through the cytosol to release stores of intracellular calcium ions (Ca2+). Montana Molecular created a series of multiplex sensors that makes it possible to simultaneously measure different components of the Gq pathway in living cells. Here we describe fluorescent sensors for DAG and PIP2that produce changes in green or red fluorescence and can be combined with one another, or with existing Ca2+ sensors, in a live-cell assay. These assays can detect multiple components of Gq signaling, simultaneously in real time, on standard fluorescent plate readers or live-cell imaging systems.
The results collected on our Biotek Synergy MX Multimode plate reader were recently validated by Jean Philippe Pin’s lab on both the Molecular Devices Flexstation and Tecan’s Infinite 500, at the Institut de Genomique Fontionnelle, CNRS.
Montana Molecular welcomes collaborations with both industry and academic labs. Please let us know if you would like information on how you can evaluate Montana Molecular’s GPCR assays in your own lab.
