cADDis: Detect cAMP in Living Cells in Real Time

  • Measure kinetic responses
  • Choose red or green fluorescent sensors
  • No cell lysis required
  • Combine with Ca2+ or DAG sensors for simultaneous pathway readout
  • BacMam delivery to primary cells, iPSCs, or standard cell lines

Our green fluorescent cADDis sensor in HEK293 cells responding to a selective agonist for the endogenous β2 adrenergic receptor (iso.)

Make Discoveries

cADDis BacMam Kits

US & Canada Prices:
5 mL … $395
10 mL … $595
30 mL … $1195
60 mL … $1795

Outside the US & Canada? Please check with regional distributors for local pricing.

Plate Reader or Microscope Compatible

Kinetics or Dose Response

Monitor Dynamic cAMP Levels

cADDis is fully reversible

Easy to Detect

Detect Gs or Gi-mediated signaling

More Information

HEK293 cells, BacMam vector

 

 

 

 

 

 

 

 

 

Protocols can be found in our main menu across the top of the page or by clicking the link below:

•  Green cADDis cAMP Protocol

•  cADDis for Gi Protocol

•  Cilia-Targeted cADDis cAMP Protocol

•  Red cADDis cAMP Protocol

Read about BacMam expression in different cell types

• Tewson, P., Martinka, S., Berlot, C., Hughes, T., Quinn, A.M, Tillo, S. Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling. SLAS 2017.

•  SLAS Conference Abstract, 2017. Direct Measurements of Gi signaling and its dynamic interplay with Gs using cADDis, a live-cell assay for cAMP with improved sensitivity.

•  Moore, B.S., Stepanchick, A.N., Tewson, P.H., Hartle, C.M., Zhang, J., Quinn, A.M., Hughes, T.E., Mirshahi, T. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics. PNAS 2016.

• Tewson, P., Martinka, S., Shaner, N.C., Hughes, T., Quinn, A.M. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen 1087057115618608, first published on December 11, 2015 as doi:10.1177/1087057115618608

In your cADDis Kit:

  • 5,10, 30, or 60 mL of Sensor
  • HDAC Inhibitor
  • Agonist Controls
  • GPCR Receptor Controls
  • Gi Kit contains active Gs

Got Questions?

We can help!