The best thing about Montana Molecular’s biosensors has been the ease of use. My lab has already been doing live cell imaging, mostly free Ca2+.  All we had to do is to infect the cells and use the same microscope set-up to gather data on PIP2, DAG and cAMP.

Vladlen Z Slepak, PhD

Director of Graduate Program Department of Molecular and Cellular Pharmacology , University of Miami School of Medicine

Graph showing the multiplex function of Montana Molecular's bright red and green fluorescent sensors.

Bright Red & Green
Fluorescent Sensors

cAMP
cGMP
DAG
PIP2

Robust Assays in Living Cells

» Easy assay steps – No cell lysis
» BacMam delivery to most cell types
» Fluorescence imaging or plate reader detection (Z’ > 0.8)
» Physiologically-relevant and cell friendly
» No forskolin or IBMX needed
» No enzymes or co-factors
» Multiplex GPCR Assays: Gs, Gq, Gi, Voltage

Simultaneous measurements of GPCR signaling in live HEK 293 cells. Green sensors in the top panels, red sensors in the bottom panels. M1 muscarinic receptor activated by carbachol.

Recent discoveries using our live cell fluorescent sensors:

P. Tewson, A.M. Quinn, T. Hughes. Dynamic Measurements of Gi Signaling in Living Cells. FASEB J. 2016 April, vol. 30 no. 1. Supplement lb478

Fluorescent Assays for Live Cell Discovery

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