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Pseudo SARS-CoV-2 D614G Mutation Red Reporter #C1120R

Baculovirus pseudotyped with SARS-CoV-2 D614G mutated spike protein that binds with ACE2 and expresses red fluorescent protein at the nucleus 24 hours after viral entry.

SARS-CoV-2 assays and reagents

Baculovirus vector pseudotyped with D614G mutated SARS-CoV-2 spike protein:

D614G Pseudo SARS-CoV-2 is a BSL-1 baculovirus  pseudotyped with D614G spike isolate. Pseudovirus enters the host cell through interactions with ACE2 and other host proteins, then delivers a reporter that expresses bright red fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host fluorescence is decreased. 

D614G PseudoSARS-CoV-2 is pseudotyped with the D614G variant of the SARS-CoV-2 spike protein. This European isolate spike protein decorates the surface of the pseudovirus, facilitating entry into cells expressing ACE2.

  • Detect host-pathogen interaction in living cells.
  • Identify therapeutic drugs that block ACE2-mediated viral entry.
  • Cost effective screening with minimal safety requirements (BSL-2).

Materials in the Kit:

• D614G Pseudo SARS CoV-2 Red NLS

• Sodium Butyrate, 500 mM in H2O

• Detailed protocol

 

This SARS-CoV-2 Reporter is currently backordered . Please contact us if interested in ordering this kit.

Product Info & Protocols

Protocol for Use

https://montanamolecular.com/fluorescent-pseudo-sars-cov-2-reporter-protocol/

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. Purchases over $600 receive free shipping.

MSDS

https://montanamolecular.com/wp-content/uploads/2020/05/Pseudo-SARS-CoV-2-MSDS-PDF.pdf

Kit Volume

5mL, 30mL, 60mL

More Info

https://montanamolecular.com/covid-19-drug-discovery/

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