#D1020G β-arrestin-GLP-1R Assay
$695.00 – $2,395.00
Kinetic beta-arrestin signaling assay through activation of GLP-1R. Monitor beta-arrestin signaling on fluorescence plate readers or imaging systems. Standard stock comes packaged in BacMam with CMV promoter, other vectors and promoters are available, please inquire!
A kinetic, real time assay for β-arrestin signaling through GLP-1R activation. The β-arrestin sensor included is a genetically-encoded biosensor packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including pancreatic islets. Measure robust signals 24 hours post transduction on standard fluorescence microscopes and automated plate readers.
Featured Posters:
- Using receptor kinetics to quantitatively measure agonist bias at G-protein coupled receptors
- Coloring β-arrestin signaling green, and G-protein signaling red, a new live cell assay for quantitatively measuring agonist bias at seven transmembrane domain receptors.
- Measuring biased agonism with a kinetic analysis platform for real time data from fluorescent biosensors
Materials in the Kit:
• Green β-arrestin biosensor BacMam
• Sodium Butyrate, 500 mM in H2O
• GLP-1 Receptor BacMam
• Exendin-4, 500 µM in DPBS
• GRK5
Additional information
| Protocol for Use | https://montanamolecular.com/beta-arrestin-assays/green-fluorescent-beta-arrestin-assays-protocol/ |
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| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| MSDS | |
| Scientific Publications | |
| More Info | |
| Kit Volume | 10mL, 30mL, 60mL |
References
cADDis cAMP Assays
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PIP2
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- P. Tewson, et al. A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. J. Biomolecular Screening 18, 2013.
DAG
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- Arttamangkul, et al.Does PKC activation increase the homologous desensitization of μ opioid receptors?British Journal of Pharmacology April 2014
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β-Arrestin
- S. Hoare, et al. Analyzing kinetic signaling data for G-protein-coupled receptors. Nature Scientific Reports July 2020.
- S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
