Reagents for SARS-CoV-2 Research
BacMam pseudoviruses and “pseudo host” vectors carrying host-cell proteins for viral entry.
Pseudo Host Products
Safe and robust tools to study SARS-CoV-2 viral entry are now available to empower discovery of compounds or antibodies that can block host infection. BacMam BSL-1 pseudoviruses “Pseudo SARS-CoV-2” and “Pseudo Host” tools reduce startup time by eliminating complicated BSL-3 safety requirements. This means that biologists and drug discovery teams can be screening within a few days, with few changes to work flow or safety/regulatory procedures. BacMam is more cost effective to use than lentivirus.
Pseudo Host Cell ACE2 can be expressed in practically any cell type to produce the surface proteins necessary for SARS-CoV-2 entry. Fluorescent ACE2 and TMPRSS2 are delivered to pseudo host cells using standard VSVG-pseudotyped BacMam.
Pseudo SARS-CoV-2 BacMam is pseudotyped with variants of the SARS-CoV-2 spike protein formed during BacMam production in Sf9 insect cells. Spike decorates the surface of the pseudovirus, facilitating entry into cells expressing ACE2 and TMPRSS2.
Check out the COVID-19 Drug Discovery page for more information.
Pseudo SARS-CoV-2 viruses are pseudotyped with either the orginal spike protein or the D614G variant.
“Original” pseudoviruses are pseudotyped with the SARS-CoV-2 spike protein (NCBI Reference Sequence: YP_009724390.1).
D614G variant pseudoviruses have the spike protein that was originally discovered in European isolates. Viruses carrying it are now found to be the dominant form in many countries (Korber et al. 2020).
Fluorescent ACE2 (Green or Red)
ACE2, normally expressed on the surface of human airway cells, interacts tightly with the SARS-CoV-2 spike protein. Red or green fluorescent ACE2 can be expressed in practically any mammalian cell type.
TMPRSS2 in BacMam facilitates controlled expression of the host serine protease that cleaves the SARS CoV-2 spike protein and enhances viral entry.
“Pseudo Host” HEK293 cells expressing fluorescent ACE2. Images acquired every 5 minutes for 5 hours on a BioTek LionHeart FX.