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Assays for GPCR Signaling: Kinetics Intro Video



Data Analysis

Time course equations for a variety of curve shapes entered into GraphPad Prism as a plug-in.

Parameters include:

    •  Initial rate of signal generation
    • Fade (desensitization) rate
    • Steady-State signal level
    • Peak level and peak time


Rise and fall to steady-state curve

Rise to steady-state curve

Quantifying GPCR Signaling and Desensitization Kinetics

Waveform data fitted to equations to quantify:

    • Signal generation rate
    • Desensitization rate
    • Signal clearance rate
    • Differences between GPCRs in signaling mechanisms and rates