The Cell Stress Assay produces a bright green fluorescent protein when the cell endures endoplasmic reticulum (ER) stress or undergoes the unfolded protein response (UPR). This robust assay can be detected on standard fluorescence plate readers or imaging systems.
New Fluorescent Tools to Monitor Cell Stress & Toxicity
Bright fluorescent assay to detect response and reversal of chemical or genetic ER stress response and UPR. Don’t settle for counting dead cells…instead, take a closer look at the biology.
- Discover a bright fluorescent assay for detecting ER-mediated stress and the UPR
- Neurotoxicity assay in iPSC-derived neurons or cardiomyocytes
- Simultaneous readouts of disrupted Ca2+signals in stressed cells
- Assay reversibility follows stress reversal
- Reveal disrupted signals that underlie neurotoxicity and neurodegeneration.
- Meet the scientists who developed the Cell Stress Assay
- Learn how the Cell Stress Assay can be a critical tool in your research
- Identify compounds that reverse cell stress
- Get results in hours not days.
- Plate reader or imaging compatible
- Get technical support fast from a friendly scientist.
Detecting Chemical Stress
Discriminate between toxic compounds in less than 8 hours.
Cells transduced with 25 μL of green cell stress sensor and activated with either thapsigargin or tunicamycin. The plot displays the percent change in fluorescence over time compared to 1 hour after addition of either drug or DMSO control.
Detecting Genetic Stress
Reach for a better understanding of neurodegeneration with a robust and reversible assay that indicates the stress pathway involved in protein misfolding and aggregation. Cells expressing Rhodopsin P23H mutation in Retinitis Pigmentosa, and SOD G85R mutation in ALS clearly indicated after just 18 hours.
Two-color Ratiometric Stress Assay
Two-color ratiometric stress biosensor: This version of the assay is designed for ratiometric measurements using the green fluorescent sensor and a constitutively expressed red fluorescent protein. Red and green fluorescent signals are both localized to the nucleus. Red fluorescence indicates all cells expressing the sensor, and green fluorescence indicates cells undergoing the stress response.
Detecting Stress Reversal
A red fluorescent nucleus turns green when the cells become stressed and back to red when cells recover, making it easy to identify therapeutic compounds that reverse ER-mediated stress and the UPR.
The cell stress assay is packaged in BacMam, a BSL-1 viral vector for efficient expression in a wide variety of cell types including primary cells, standard cell lines, and iPSC derived cells. In the videos below we show how the cell stress assay responds to stress and toxicity in iPSC neurons and cardiomyocytes.
Stress and toxicity assay in iPSC derived neurons
Stress and toxicity assay in iPSC cardiomyocytes
- K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Front. Cell. Neurosci., December 2019 .
- K. Harlen, et al. New Fluorescent Tools to Monitor Cell Stress and Toxicity. SLAS Poster. 2018.
- T. Hughes, et al. Measuring Signal Transduction in Axiogenesis Cardiomyocytes. Poster. 2018.
- H. Yoshida, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001.
BioTek Application Note
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