Drug Discovery & Screening

Profile compounds and uncover mechanisms-of-action with fluorescent biosensors. Accelerate discovery with flexible, customizable BacMam expression vectors

While traditional endpoint assays provide a single data point, our live-cell fluorescent biosensors deliver a high-resolution view of signaling events. By monitoring responses continuously, you capture the nuances of compound behavior that single-time point assays miss – making these assays ideal for secondary screening and compound characterization. This approach enables detailed profiling of confirmed hits, including signaling kinetics, mechanism-of-action insights, potency and efficacy estimates, and differentiation of compounds based on pathway dynamics and functional selectivity.

 

• Multipathway readouts: Measure cAMP, DAG, Ca²⁺, and arrestin recruitment responses on a single platform

• Pharmacological Insights: Waveform analysis of signaling kinetics provides precise estimates of initial response rate, potency, efficacy, signal duration, and receptor desensitization.

• Detailed Compound Profiling: Accurately characterize and differentiate the activity of agonists, antagonists, partial agonists, and more – all in a single assay.

Detecting Persistent Signaling

Measure prolonged signaling using fluorescent biosensors in living cells. The cADDis cAMP sensor enables continuous monitoring of GPCR signaling for up to 24 hours. Download a poster below.

Express GPCRs, ion channels, and other therapeutic targets in mammalian cells— across standard screening lines, iPSC-derived, and primary cultures—supporting preclinical drug discovery from HTS through detailed MOA studies.

• Titratable Expression: Precisely control receptor or target density to match physiological conditions, uncover partial agonists, and explore how density affects EC50.

• Massive Payload: Deliver large genes, multi-subunit proteins, cell-specific promoters, or multi-gene combinations with a 38kb capacity.

• Bespoke Solutions: Leverage our expertise to design Custom Bacmam vectors tailored to your experiments – including custom gene expression, species orthologs, splice variants, mutations, fluorescent or genetic tags, multi-gene expression cassettes, or CRISPR/Cas systems.

Save time and resources while harnessing BacMam’s versatile capabilities. Contact our team to create the tools your research requires.

Montana Molecular specializes in mechanism-of-action pharmacology, using BacMam and in-house biosensor platforms to deliver fast, reliable, and detailed compound analyses.

• Multi-Species Profiling: Compare activity across human and ortholog targets (mouse, rat, NHP).

• Kinetic Assays: Continuous-read assays reveal activators, inhibitors, allosteric modulators, bias, and time-dependent effects not observable in endpoint formats.

• Tunable Receptor Levels: Adjust expression to detect partial agonists and fine-tune signaling sensitivity.

• Mechanistic Insights: Measure GPCR signaling kinetics, desensitization, and complex multi-step responses—including washout experiments and sequential compound additions—to uncover detailed mechanisms of action.

By leveraging BacMam for rapid assay optimization, Montana Molecular dramatically shortens timelines compared to stable cell lines – delivering presentation-ready results faster.