Application Notes
Real Time, Multiday Cell Stress Assay
Quantifying the unfolded protein response using a nontoxic genetically-encoded fluorescent sensor, live-cell imaging, and label-free cellular impedance.
Dual Channel Kinetic Assays for Detecting Ligand Bias at GPCRs
- Genetically-encoded, single-color fluorescent biosensor detects β-arrestin signaling in living cells.
- β-arrestin sensor multiplexed with Gq sensor simultaneously detects arrestin and G-protein signaling.
- Kinetic analysis of arrestin and G-protein signaling on the CLARIOstar® reliably detects agonist bias.
Real-Time Detection of Gs and Gi Signaling in Living Cells on a BMG CLARIOstar
- Genetically-encoded biosensors from Montana Molecular allow for detection of GPCR signaling.
- Robust Z′ measurements, kinetic readouts, and dose response
Simultaneous detection of GPCR second messengers in living cells
- Quantification of Gq signaling in living cells on BMG LABTECH’s CLARIOstar® microplate reader
- Multiplexing to monitor signal transduction cascades using kinetic readouts
Characterizing GPCR Activaton using Live Cell Imaging- Biotek Instruments
Fluorescent dyes and biosensors can be used to monitor changes in second messenger levels, including Ca2+ and cAMP, in response to GPCR activation. Here we describe a live-cell imaging based approach to detect GPCR activation using the Lionheart FX automated imager and Gen5 image analysis software. This method provides a large assay window and improved sensitivity over methods relying on total fluorescence intensity measurements.
