Live Cell PIP2 Assays

Order PIP2 Assays

The sensor fluoresces in either green or red.

Choose Green PIP2Choose Red PIP2

Green or Red PIP2 Assays

  • Bright fluorescent PIPsensor
  • Real time kinetic assay
  • Robust expression in variety of cell types

The PIPassay is a genetically encoded biosensor for PIP2 with a green or red dimerization-dependent fluorescent protein. These downward sensors start bright and decrease fluorescence as PIP2 is hydrolyzed to produce DAG and IP3.

PIP2 Assay Performance

  • Genetically-encoded for low toxicity
  • PIP2 sensor and controls are packaged in BSL1 BacMam vector for efficient expression in variety of cells
  • Single channel readout can be combined with additional GPCR assays
  • Real time kinetic readout

Multiplex GPCR Assays

Combine red and green sensors to measure multiple GPCR pathways simultaneously

Simple Protocol

  • No cell lysis
  • Single channel readout
  • BSL-1 Vector
  • Controlled expression levels

Order PIP2 Assays

The sensor fluoresces in either green or red.

Choose Green PIP2Choose Red PIP2