PIP2 Assay Kit

Browse all PIP2 Assays

Red or Green PIP2 Assays

  • Bright fluorescent PIP2 sensor
  • Real time kinetic assay
  • Robust expression in variety of cell types

Simple Protocol

  • No cell lysis
  • Single channel readout
  • BSL-1 Vector
  • Controlled expression levels

Multiplex GPCR Assays

Combine red and green sensors to measure multiple GPCR pathways simultaneously

The PIPassay is a genetically encoded biosensor for PIP2 with a red or green dimerization-dependent fluorescent protein. These downward sensors start bright and decrease fluorescence as PIP2 is hydrolyzed to produce DAG and IP3.

PIP2 Assay Advantages:

  • Genetically encoded for low toxicity
  • PIP2 sensor and controls in BSL1 BacMam vector for efficient expression in variety of cells
  • Single channel readout can be combined with additional GPCR assay
  • Real time kinetic readout 

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