Live Cell PIP2 Assays
Green or Red PIP2 Assays
- Bright fluorescent PIP2 sensor
- Real time kinetic assay
- Robust expression in variety of cell types
The PIP2 assay is a genetically encoded biosensor for PIP2 with a green or red dimerization-dependent fluorescent protein. These downward sensors start bright and decrease fluorescence as PIP2 is hydrolyzed to produce DAG and IP3.
PIP2 Assay Performance
- Genetically-encoded for low toxicity
- PIP2 sensor and controls are packaged in BSL1 BacMam vector for efficient expression in variety of cells
- Single channel readout can be combined with additional GPCR assays
- Real time kinetic readout
Multiplex GPCR Assays
Combine red and green sensors to measure multiple GPCR pathways simultaneously
Simple Protocol
- No cell lysis
- Single channel readout
- BSL-1 Vector
- Controlled expression levels
Recent Publications
- L.Liu, et al. Gαq sensitizes TRPM8 to inhibition by PI(4,5)P2 depletion upon receptor activation. J.Neurosci. May 2019.
- , and Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor Molecular Pharmacology Nov. 2018
- Q. Wang, et al. Regulator of G protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion. FASEB J. Jul. 7,2017.
- Y. Ding, et al. Ratiometric biosensors based on dimerization dependent fluorescent protein exchange. Nature Methods, 2015.
- P. Tewson, et al. A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. J. Biomolecular Screening 18, 2013.
