Montana Molecular develops single-color genetically encoded biosensors for detection of signal transduction pathways in living cells. Our sensors fluoresce in either green or red, and they can be combined with one another for the simultaneous detection of multiple analytes.
- Detect kinetic Gs, Gi, and Gq mediated responses in living cells
- Combine multiple assays in the same cell population
- Express sensors and run assays in disease relevant cell types
- Detect fluorescence on imaging systems or automated plate readers (Z’>0.8)
Detect Gs mediated cAMP fluctuations with the cADDis cAMP assay.
Our cADDis cAMP sensor can be also used to detect Gi mediated responses.
Detect Gq mediated responses using R-GECO Ca2+, DAG, and PIP2 assays.
cADDis cAMP Assay
R-GECO Ca2+ Assay
Continuous Multiplex Measurements
Assays in Primary Cultures or iPSCs
Detect GPCR mediated responses in cells relevant to your disease, drug target, or biology of interest. Our BacMam-packaged sensors have been used in neurons, cardiomyoctes, islets, and many more primary and iPSC derived cells! Alternate promoters or viral vectors are available by request. The BacMam Transducible Cells page lists some publications showing disease-relevant cells transduced with BacMam. Pictured, at right, are the cADDis cAMP Assay in primary striatal neurons and the R-GECO calcium assay in nCardia’s Cor.4u cardiomyocytes.
Fluorescent beta-arrestin assays to detect arrestin recruitment at specific GPCRs are available as well. They contain the Arrestin sensor, GPCR and relevant GRK in BacMam, positive control agonist, and sodium butyrate. GPCRs include Angiotensin II (AT1R), Vasopressin (V2R), GLP-1, Mu Opioid (OPRM1), and beta2-adrenergic (B2AR) receptors. We are continuing to validate new receptors, if you do not see your receptor of interest please send us an email!