DAG: Live Cell Diacylglycerol Assays
Bright Fluorescent Biosensors for Diacylglycerol
- Genetically-encoded for low toxicity
- Plate reader or imaging system compatible
- Choose red or green fluorescence
DAG Assay Performance
Off the shelf kits include the DAG sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Easily detectable on plate readers or imaging systems
- Consistent well-to-well expression
- High signal-to-noise ratio
- Combine red and green sensors to measure multiple GPCR pathways simultaneously
- Red or green sensor version
- Single channel, non-FRET signal
Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.
A Robust & Versatile DAG Assay Kit
Which Sensor is Right for You: Up or Down?
“We have been delighted to work with MontanaMolecular ever since we noticed a publication 5 years ago concerning DAG sensors that MM had created and that we employed subsequently to examine DAG signaling in cardiac cells. We were impressed first by the friendly and scientific way in which MM scientists interacted with us. Then, we were impressed how MM scientists helped us generously to reach our goals to monitor DAG changes and to consider new approaches to develop and exploit sensing molecules. Recently, MM has helped us design and build BaculoViruses to express and manipulate important proteins in a variety of cells that interest us. Again, we have been delighted by the competence of MM scientists, the reliability of the products we received, and the pleasure that MM scientists show in accomplishing great science in collaboration. It our genuine pleasure to recommend MM to all our colleagues with similar scientific interests and needs.”
“Assay is working beautifully with cardiomyocytes, showing a strong and immediate response.”
“Expression in human islets is pretty superb, after 18 hours of incubation. An excellent probe!”
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- P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 2015. Request Full Text PDF
- C. Peters, et al. Simultaneous detection of GPCR second messengers in living cells. BMG LabTech Dec. 2016.
Increase your understanding of drug effects and GPCR biology with bright fluorescent assays for Gs, Gi, and Gq signaling in living cells.