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“We have been delighted to work with MontanaMolecular ever since we noticed a publication  5 years ago concerning DAG sensors that MM had created and that we employed subsequently to examine DAG signaling in cardiac cells. We were impressed first by the friendly and scientific way in which MM scientists interacted with us. Then, we were impressed how MM scientists helped us generously to reach our goals to monitor DAG changes and to consider new approaches to develop and exploit sensing molecules.   Recently, MM has helped us design and build BaculoViruses to express and manipulate important proteins in a variety of cells that interest us.  Again, we have been delighted by the competence of MM scientists, the reliability of the products we received, and the pleasure that MM scientists show in accomplishing great science in collaboration.  It our genuine pleasure to recommend MM to all our colleagues with similar scientific interests and needs.”

Donald W. Hilgemann, Ph.D.

Professor, Roy & Christine Sturgis Chair in Biomedical Research, UT Southwestern Medical Center

Bright Fluorescent Biosensors for Diacylglycerol

Downward DAG starts out bright and decreases in fluorescence when diacylglycerol levels increase. This assay is easily detected on fluorescence plate readers and helps with imaging cells that have low expression efficiency.

Upward DAG starts out dim and increases in fluorescence when when diacylglycerol levels increase. This DAG assay is easily detected on fluorescence plate readers or imaging systems.

DAG Assay Performance

Off the shelf kits include the DAG sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.

  • Easily detectable on plate readers or imaging systems
  • Consistent well-to-well expression
  • High signal-to-noise ratio

Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.

In HEK 293 cells over-expressing the P2Y11  receptor, ATP produces changes in both DAG and Ca2+, consistent with activation of Gq-coupled GPCR signaling. UTP produces no DAG response, indicating that the Ca2+ change is independent of Gq.

Multiplex Assays

A Robust & Versatile DAG Assay Kit

  • Detect diacylglycerol increases in real time
  • Screen Gq coupled GPCRs in living cells
  • Viral vector delivery to practically any cell type
  • Target to microdomains or specific cells in mixed cultures

Simple Protocol

  • Minimal liquid handling
  • No cell lysis
  • 96 or 384 well compatible

“Assay is working beautifully with cardiomyocytes, showing a strong and immediate response.”

Kit Werley

Harvard University / Q-State Biosciences

“Expression in human islets is pretty superb, after 18 hours of incubation. An excellent probe!” 

Dr. Andrei Tarasov

Oxford Center for Diabetes

Recent Publications