DAG: Live Cell Diacylglycerol Assays
Bright Fluorescent Biosensors for Diacylglycerol
- Genetically-encoded for low toxicity
- Plate reader or imaging system compatible
- Choose red or green fluorescence
DAG Assay Performance
Off the shelf kits include the DAG sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
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- Easily detectable on plate readers or imaging systems
- Consistent well-to-well expression
- High signal-to-noise ratio
Multiplex Assays
- Combine red and green sensors to measure multiple GPCR pathways simultaneously
- Red or green sensor version
- Single channel, non-FRET signal
Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.
A Robust & Versatile DAG Assay Kit
- Detect diacylglycerol increases in real time
- Screen Gq coupled GPCRs in living cells
- Viral vector delivery to practically any cell type
- Target to microdomains or specific cells in mixed cultures
Simple Protocol
- Minimal liquid handling
- No cell lysis
- 96 or 384 well compatible
Which Sensor is Right for You: Up or Down?
Testimonials
“We have been delighted to work with MontanaMolecular ever since we noticed a publication 5 years ago concerning DAG sensors that MM had created and that we employed subsequently to examine DAG signaling in cardiac cells. We were impressed first by the friendly and scientific way in which MM scientists interacted with us. Then, we were impressed how MM scientists helped us generously to reach our goals to monitor DAG changes and to consider new approaches to develop and exploit sensing molecules. Recently, MM has helped us design and build BaculoViruses to express and manipulate important proteins in a variety of cells that interest us. Again, we have been delighted by the competence of MM scientists, the reliability of the products we received, and the pleasure that MM scientists show in accomplishing great science in collaboration. It our genuine pleasure to recommend MM to all our colleagues with similar scientific interests and needs.”
“Assay is working beautifully with cardiomyocytes, showing a strong and immediate response.”
“Expression in human islets is pretty superb, after 18 hours of incubation. An excellent probe!”
Recent Publications
- G. Sanchez, et al. Coincident Regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid Receptors Opposes Opioid-Mediated Antinociception. Molecular Pharmacology. December 2022.
- Z. Miller, et al. Lidocaine Induces Apoptosis in Head and Neck Squamous Cell Carcinoma Cells Through Activation of Bitter Taste Receptor T2R14. bioRxiv. November 2022.
- M. Doepner, et al. Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling. Science Advances. September 2022.
- A. Kim, et al. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes. eLife. November 2021. (bioRxiv)
- S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
- E. Maguire, et al. The Alzheimer's disease protective P522R variant of PLCG2, consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function. bioRxiv. April 2020.
- L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.
- S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
- C Deisl et al. Hypertrophy of human embryonic stem cell–derived cardiomyocytes supported by positive feedback between Ca2+ and diacylglycerol signals. Pflügers Archiv – European Journal of Physiology Jun. 2019
- Herranz, Gonzalo et al. Protein Kinase C δ Regulates the Depletion of Actin at the Immunological Synapse Required for Polarized Exosome Secretion by T Cells. Frontiers in Immunology Apr. 2019
- Ohno, et al. Dynamics of Presynaptic Diacylglycerol in a Sensory Neuron Encode Differences between Past and Current Stimulus Intensity. Cell Reports, Vol. 20, Issue 10. Sept. 2017
- Q. Wang, et al. Regulator of G protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion. FASEB J. Jul. 7,2017.
- Shigeto, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clinical Invest. 2015. doi:10.1172/JCI81975.
- Xingjuan, et al. PKC-dependent Phosphorylation of the H1 Histamine Receptor Modulates TRPC6 Activity. Cells. 2014.
- P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 2015. Request Full Text PDF
- C. Peters, et al. Simultaneous detection of GPCR second messengers in living cells. BMG LabTech Dec. 2016.
GPCR Biology
Increase your understanding of drug effects and GPCR biology with bright fluorescent assays for Gs, Gi, and Gq signaling in living cells.
