“We have been delighted to work with MontanaMolecular ever since we noticed a publication 5 years ago concerning DAG sensors that MM had created and that we employed subsequently to examine DAG signaling in cardiac cells. We were impressed first by the friendly and scientific way in which MM scientists interacted with us. Then, we were impressed how MM scientists helped us generously to reach our goals to monitor DAG changes and to consider new approaches to develop and exploit sensing molecules. Recently, MM has helped us design and build BaculoViruses to express and manipulate important proteins in a variety of cells that interest us. Again, we have been delighted by the competence of MM scientists, the reliability of the products we received, and the pleasure that MM scientists show in accomplishing great science in collaboration. It our genuine pleasure to recommend MM to all our colleagues with similar scientific interests and needs.”
DAG Assay Performance
Off the shelf kits include the DAG sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Easily detectable on plate readers or imaging systems
- Consistent well-to-well expression
- High signal-to-noise ratio
In HEK 293 cells over-expressing the P2Y11 receptor, ATP produces changes in both DAG and Ca2+, consistent with activation of Gq-coupled GPCR signaling. UTP produces no DAG response, indicating that the Ca2+ change is independent of Gq.
- Combine red and green sensors to measure multiple GPCR pathways simultaneously
- Red or green sensor version
- Single channel, non-FRET signal
- Minimal liquid handling
- No cell lysis
- 96 or 384 well compatible
“Assay is working beautifully with cardiomyocytes, showing a strong and immediate response.”
“Expression in human islets is pretty superb, after 18 hours of incubation. An excellent probe!”
- C Deisl et al. Hypertrophy of human embryonic stem cell–derived cardiomyocytes supported by positive feedback between Ca2+ and diacylglycerol signals. Pflügers Archiv – European Journal of Physiology Jun. 2019
- Herranz, Gonzalo et al. Protein Kinase C δ Regulates the Depletion of Actin at the Immunological Synapse Required for Polarized Exosome Secretion by T Cells. Frontiers in Immunology Apr. 2019
- Ohno, et al.Dynamics of Presynaptic Diacylglycerol in a Sensory Neuron Encode Differences between Past and Current Stimulus Intensity. Cell Reports, Vol. 20, Issue 10. Sept. 2017
- Shigeto, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation.J Clinical Invest. 2015. doi:10.1172/JCI81975.
- P. Rorsman, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation J Clinical Investigation Nov. 2015
- Arttamangkul, et al. Does PKC activation increase the homologous desensitization of μ opioid receptor? British Journal of Pharmacology.Jan. 2015.
- Xingjuan, et al. PKC-dependent Phosphorylation of the H1 Histamine Receptor Modulates TRPC6 Activity.Cells. 2014.
- P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 2015. Request Full Text PDF
- J. Williams, et al. Does PKC activation increase the homologous desensitization of μ opioid receptors? British Journal of Pharmacology April 2014
- Tewson, et al. Simultaneous Detection of Ca2+and Diacylglycerol Signaling in Living Cells.PLoS One. 2012.
- C. Peters, et al. Simultaneous detection of GPCR second messengers in living cells. BMG LabTech Dec. 2016.