Part 1: Transduction
Part 2: Plate Reader set-up and Experiment
Sensor Expression and Function
Examples of cAMP, DAG, PIP2, Calcium, Voltage, and Beta-arrestin sensors expressing in living cells including HEK293s, cardiomyocytes, and neurons.
Simultaneous measurements of GPCR signaling in live HEK 293 cells. Green sensors in the top panels, red sensors in the bottom panels.
Red fluorescent R-GECO Calcium sensor in Ncardia (formerly Axiogenesis) Cor.4u iPSC derived cardiomyocytes.
Visualizing electrical activity in cells using GFP-based voltage probe ArcLight