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Video Protocols


Quantifying Kinetics of GPCR Signaling

Assays for GPCR Signaling Kinetics

Fluorescent biosensors for GPCR signaling kinetics – Dr. GPCR 2020

Biased Agonism made simple – Pharmechanics

Cell Stress cardiotoxicity assay in Ncardia Cor.4u iPSC derived cardiomyocytes

Live-cell detection of cell stress and toxicity in Ncardia Peri.4u iPSC derived neurons

New Fluorescent Tools to Examine Cell Stress and Toxicity

Live Cell Assays in Cardiomyocytes

Sensor Expression and Function

Examples of cAMP, DAG, PIP2, Calcium, Voltage, and Beta-arrestin sensors expressing in living cells including HEK293s, cardiomyocytes, and neurons.

Simultaneous measurements of GPCR signaling in live HEK 293 cells. Green sensors in the top panels, red sensors in the bottom panels.

Green fluorescent cADDis sensor in HEK293 cells responding to isoproterenol

Green fluorescent cyclic GMP sensor in HEK293 cells responding to Sodium Nitroprusside (SNP).

Red fluorescent R-GECO Calcium sensor in Ncardia (formerly Axiogenesis) Cor.4u iPSC derived cardiomyocytes.

Visualizing electrical activity in cells using GFP-based voltage probe ArcLight