G-Protein Coupled Receptors (GPCRs) are one of the largest receptor families in the genome and are essential for the healthy function of nearly every organ in the body. GPCRs are also important targets for therapeutic drugs. Increase your understanding of drug effects and GPCR biology with bright fluorescent assays in living cells.
Simultaneous detection of GPCR second messengers in living HEK 293 cells; By combining red and green sensors in a single transduction step, changes in two second messengers can be detected simultaneously. Green sensors in the top panels, red sensors in the bottom panels. M1 muscarinic receptor activated by carbachol.
GPCR Assays in Living Cells
- Reliably detect Gi, Gs, Gq-coupled GPCR signals.
- No enzymes, cofactors or cell lysis.
- Measure single timepoints or kinetic responses.
- Red and/or green fluorescent sensors for cAMP, DAG, Arrestin, Ca2+, PIP2
- Understand ligand bias with multiplex measurements in a single assay.
- Monitor fluorescence changes on automated plate readers or imaging systems.
- BacMam vector for efficient delivery to disease-relevant cells.
- Compatible with use in stable lines.
- Get a quote for AAV or lenti vectors
cADDIs is a simple and effective assay for detecting cAMP changes in living cells. cADDis is available as decreasing in fluorescence (Down cADDis) or increasing in fluorescence (Up cADDis). Use cADDis to detect the production of cAMP via Gs-coupled GPCRs or inhibition of cAMP production via Gi-coupled GPCRs.