GPCR Biology

G-Protein Coupled Receptors (GPCRs) are one of the largest receptor families in the genome and are essential for the healthy function of nearly every organ in the body. GPCRs are also important targets for therapeutic drugs. Increase your understanding of drug effects and GPCR biology with bright fluorescent assays in living cells.

Simultaneous detection of GPCR second messengers in living HEK 293 cells; By combining red and green sensors in a single transduction step, changes in two second messengers can be detected simultaneously. Green sensors in the top panels, red sensors in the bottom panels. M1 muscarinic receptor activated by carbachol.

GPCR Assays in Living Cells

 

Monitor cAMP in Living Cells with cADDis

cADDis Assays

cADDIs is a simple and effective assay for detecting cAMP changes in living cells. cADDis is available as decreasing in fluorescence (Down cADDis) or increasing in fluorescence (Up cADDis). Use cADDis to detect  the production of cAMP via Gs-coupled GPCRs or inhibition of cAMP production via Gi-coupled GPCRs.

Monitor Ca2+ in Living Cells with GECO

GECO Calcium Assays

GECO is a simple and effective assay for detecting Ca2+ changes in living cells

Monitor DAG in Living Cells

DAG Assays

DAG biosensors are available as decreasing in fluorescence (Down DAG) or increasing in fluorescence (Up DAG). Both versions indicate increases in diacylglycerol in living cells.

Monitor PIP2 in Living Cells

PIP2 Assays