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Live Cell ß-Arrestin Assays

Order ß-Arrestin Assays for specific GPCRs

Choose your receptor

A genetically-encoded fluorescent arrestin assay that detects changes in arrestin recruitment in real time.

  • Detect agonist bias in living cells
  • Measure kinetic cellular responses
  • Monitor Arrestin and G-limb signaling using the same modality
  • Detect responses simultaneously or in parallel

Receptor-Specific Kits

Kits are available to detect arrestin recruitment at specific GPCRs. They include the Arrestin sensor (your choice of the Angiotensin AT1, Vasopressin V2, or GLP-1 receptor in BacMam), plus a positive control agonist and sodium butyrate. We are continuing to validate new receptors, if you do not see your receptor of interest please send us an email!

Angiotensin AT1 Receptor

Vasopressin V2 Receptor

GLP-1 Receptor

Detecting Agonist Bias

  • Easily capture signaling kinetics

  • Make direct comparisons of arrestin and G-limb activity

    • Monitor responses in parallel plates using the same assay modality
    • Compare green Arrestin and red G-limb responses in the same well
  • Monitor the time course of GPCR activity in a single assay

  • Use validated, intuitive Pharmechanics analysis to assess bias with kinetic data

Green Calcium sensor and green Arrestin sensor ran in parallel plates – AT1R

Red DAG sensor and green Arrestin sensor multiplex in the same wells – AT1R

cADDis cAMP Assay

DAG Assay

PIP2 Assay

GECO Ca2+ Assay

Simple protocol:

Provided online is a simple assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.

Beta Arrestin Assay Posters

Watching Biased Agonism at GPCRs in Real Time

Coloring Beta-arrestin signaling green, and G-protein signaling red, a new live cell assay for quantitatively measuring agonist bias at seven transmembrane domain receptors.

Measuring biased agonism with a kinetic analysis platform for real time data from fluorescent biosensors