cADDis: Live Cell cAMP Assay Kit
Screening in disease relevant cells
Genetically encoded sensor packaged in a variety of vectors for robust expression in any cell type.
Combine red and green sensors to measure multiple GPCR pathways simultaneously
A robust and versatile cAMP assay kit:
cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.
- Screen Gs or Gi-coupled GPCRs in living cells
- Viral vector delivery to practically any cell type
- Target to microdomains or specific cells in mixed cultures
cADDis cAMP assay performance:
Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficiently deliver to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Easily detectable on plate readers or imaging systems.
- Controllable expression in BacMam vector.
- High signal to noise ratio
Provided online is a simple cAMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.
- Text and video protocol online
- No lysis or complex liquid handling
- Single channel, non-FRET signal
- X. Chen, et al. Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling. Scientific Reports Aug. 2018.
- N. H. Wray, et al. NMDAR-independent, cAMP-dependent antidepressant actions of ketamine. Molecular Psychiatry. April 2, 2018.
- P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 11, 2015. Request Full Text PDF
- P. Tewson, et al. Assay for Detecting Gαi-Mediated Decreases in cAMP in Living Cells. SLAS. July 10, 2018. Request Full Text PDF
- Y.A.B. Sierra, et al. Potassium channel-based optogenetic silencing. Nature Communications Nov. 5 2018.
- M. Thomas, et al. Toward a High Throughput Screen for Voltage Sensors. 2018.
- B.S. Moore, et al. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics.
PNAS Nov. 15, 2016.