cADDis: Live Cell cAMP Assays

Order cADDis cAMP Assays

The sensor fluoresces in either green or red.

Choose Green cADDisChoose Red cADDis

Detect Gs or Gi signaling with cADDis, our cAMP Assay

Downward cADDis starts out bright and decreases in fluorescence when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers and helps with imaging cells that have low expression efficiency.

Upward cADDis starts out dim and increases in fluorescence when when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers or imaging systems.

cADDis cAMP Assay Performance

Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.

  • Easily detectable on plate readers or imaging systems
  • Controllable expression in BacMam vector
  • High signal-to-noise ratio

Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.

Multiplex Assays

  • Combine red and green sensors to measure multiple GPCR pathways simultaneously

A Robust and Versatile cAMP Assay Kit:

cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.

Simple protocol:

Provided online is a simple cAMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.

Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.

It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.

 

 

Recent Publications

 

cADDis – cAMP Assay Posters

Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling

PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells

Order cADDis cAMP Assays

The sensor fluoresces in either green or red.

Choose Green cADDisChoose Red cADDis