cADDis: Live Cell Assays for cAMP
Detect Gs or Gi signaling with cADDis, our cAMP Assay
- Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor
- Plate reader or imaging system compatible
cADDis cAMP Assay Performance
Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Easily detectable on plate readers or imaging systems
- Controllable expression in BacMam vector
- High signal-to-noise ratio
Measure cAMP through Gs or Gi Signaling in Real Time with a Single Sensor
Multiplex Assays
- Combine red and green sensors to measure multiple GPCR pathways simultaneously
Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.
Simple Protocol
- Minimal liquid handling
- No cell lysis
- 96 or 384 well compatible
- Single channel, non-FRET signal
A Robust & Versatile cAMP Assay Kit
- cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.
- Screen Gs or Gi-coupled GPCRs in living cells
- Robust expression in practically any cell type
- Target to microdomains or specific cells in mixed cultures
Which Sensor is Right for You: Up or Down?
Downward cADDis starts out bright and decreases in fluorescence when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers and helps with imaging cells that have low expression efficiency.
Upward cADDis starts out dim and increases in fluorescence when when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers or imaging systems.
Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.
It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.
Recent Publications
- E. Porpiglia, et al. Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration. Cell Stem Cell. December 2022. (bioRxiv)
- J. Janetzko, et al. Membrane phosphoinositides regulate GPCR-β-arrestin complex assembly and dynamics. Cell. November 2022.
- N. Saito, et al. Gαs-Coupled CGRP Receptor Signaling Axis from the Trigeminal Ganglion Neuron to Odontoblast Negatively Regulates Dentin Mineralization. biomolecules. November 2022.
- J. Xu, et al. An evolutionarily conserved olfactory receptor is required for sex differences in blood pressure. bioRxiv. November 2022.
- B. Barsi-Rhyne, et al. Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling. eLife. October 2022. (bioRxiv)
- S. A. Dai, et al. State-selective modulation of heterotrimeric Gαs signaling with macrocyclic peptides. Cell. September 2022.
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. Science Advances. September 2022. (bioRxiv)
- E. Blythe, M. von Zastrow. A discrete mode of endosomal GPCR signaling that does not require β-arrestins. bioRxiv. September 2022.
- ER McGlone, et al. Hepatocyte cholesterol content modulates glucagon receptor signaling. Molecular Metabolism. September 2022.
- J. Xu, J. Pluznick. Key amino acids alter activity and trafficking of a well-conserved olfactory receptor. Cell Physiology. June 2022.
- C. Zhang, et al. A brainstem circuit for nausea suppression. Cell Reports. June 2022.
- J. Hansen, et al. A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. EMBO Reports. June 2022.
- S. Ansari, et al. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv. May 2022.
- S. Bitsi, et al. Divergent acute versus prolonged in vivo GLP-1R responses in β-arrestin 2-deleted primary beta cells. bioRxiv. April 2022.
- Y. Mizobuchi, et al. Ketamine Improves Desensitization of μ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine. biomolecules. March 2022.
- B. Polacco, et al. Profiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors. bioRxiv. March 2022.
- D. Lovinger, et al. Local modulation by presynaptic receptors controls neuronal communication and behavior. Nature Reviews Neuroscience. February 2022.
- F. De Logu, et al. Schwann cell endosome CGRP signals elicit peri orbital mechanical allodynia in mice. Nature Communications. February 2022.
- A. Lutas, et al. History-dependent dopamine release increases cAMP levels in most basal amygdala glutamatergic neurons to control learning. Cell Reports. January 2022.
- G. Sancar, et al. FGF1 and insulin control lipolysis by convergent pathways. Cell Metabolism. January 2022.
- S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
Posters: cADDis cAMP Assay
Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
GPCR Biology
Increase your understanding of drug effects and GPCR biology with bright fluorescent assays for Gs, Gi, and Gq signaling in living cells.
cADDis cAMP Assay Kits
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#D0200G Green Down cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200G Green Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#X0200G Green Gi cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200R Red Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#D0231G Green Membrane-Targeted FMP cADDis cAMP
$695.00 – $1,495.00 -
#D0241G Green Membrane-Targeted fS15 cADDis cAMP
$695.00 – $1,495.00 -
#U0241R Red Membrane-Targeted fS15 cADDis cAMP
$695.00 – $1,495.00 -
#U0205G Green Upward cADDis cAMP (Promoter CAG)
$695.00 – $2,395.00
