cADDis: Live Cell Assays for cAMP
Detect Gs or Gi signaling with cADDis, our cAMP Assay
- Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor
- Plate reader or imaging system compatible
Measure cAMP through Gs or Gi Signaling in Real Time with a Single Sensor
- Quantify kinetics of cAMP signaling in living cells.
- Measure cAMP for many hours with minimal loss of sensor signal.
- Robust expression in practically any cell type.
Quantify cAMP Kinetics Precisely
- Changes over time reflect biological signaling mechanisms (rather than technical artifacts like sensor decay).
- Quantify signaling mechanisms by curve fitting time course data (see here for example report).
- In the example below we quantify the signal generation rate, signal decline resulting from desensitization, and late stage decline (likely resulting from peptide ligand degradation).
Your endpoint assays are missing important information. Measuring kinetics with our live-cell assays can reveal it.
- Signaling dynamics of multiple drug compounds
- Different dynamics of cAMP generation by β2 adrenergic receptor agonists
- Clenbuterol and salbutamol generate cAMP more slowly and clenbuterol desensitizes receptor more slowly
A Robust & Versatile cAMP Assay Kit
- cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time. Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Screen Gs or Gi-coupled GPCRs in living cells
- Robust expression in practically any cell type
- Easily detectable on plate readers or imaging systems
- Controllable expression in BacMam viral vector
- High signal-to-noise ratio
- Target to microdomains or specific cells in mixed cultures
GPCR Assay Services
Go from hit to lead faster with compound profiling assays that capture GPCR signaling dynamics in living cells. Our unique biosensor-based discovery platform quantifies multiple signaling parameters in real time to enable decisions based on clinically-relevant data. Our fast turnaround time helps reduce the time to the clinic. We will perform experiments to advance your projects and programs, for example by optimizing assays, profiling compounds, exploring mechanisms and kinetically characterizing signaling dynamics.
Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.
It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.
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- ER McGlone, et al. Hepatocyte cholesterol content modulates glucagon receptor signaling. Molecular Metabolism. September 2022.
Posters: cADDis cAMP Assay
Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
Increase your understanding of drug effects and GPCR biology with bright fluorescent assays for Gs, Gi, and Gq signaling in living cells.