cADDis: Live Cell cAMP Assay Kit

Order cAMP Assays

Detect Gs or Gi signaling with cADDis, our cAMP Assay

  • Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor
  • Plate reader or imaging system compatible

Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.

Multiplex Assays

cADDis cAMP Assay Performance

Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.

  • Easily detectable on plate readers or imaging systems
  • Controllable expression in BacMam vector
  • High signal-to-noise ratio

A Robust and Versatile cAMP Assay Kit:

cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.

  • Screen Gs or Gi-coupled GPCRs in living cells
  • Robust expression in practically any cell type
  • Target to microdomains or specific cells in mixed cultures

Simple protocol:

Provided online is a simple cAMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.

Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.

It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.

 

 

Recent Publications

 

cADDis – cAMP Assay Posters

Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling

PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells

Order cADDis cAMP Assay Kits