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cADDis: Live Cell Assays for cAMP

Detect Gs or Gi signaling with cADDis, our cAMP Assay

  • Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor
  • Plate reader or imaging system compatible

cADDis cAMP Assay Performance

Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.


  • Easily detectable on plate readers or imaging systems
  • Controllable expression in BacMam vector
  • High signal-to-noise ratio

Measure cAMP through Gs or Gi Signaling in Real Time with a Single Sensor

Multiplex Assays

  • Combine red and green sensors to measure multiple GPCR pathways simultaneously

Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.

Simple Protocol

    • Minimal liquid handling
    • No cell lysis
    • 96 or 384 well compatible
    • Single channel, non-FRET signal

A Robust & Versatile cAMP Assay Kit

  • cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.

Which Sensor is Right for You: Up or Down?

Downward cADDis starts out bright and decreases in fluorescence when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers and helps with imaging cells that have low expression efficiency.

Upward cADDis starts out dim and increases in fluorescence when when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers or imaging systems.

Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.

It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.



Recent Publications

Click here for a complete list of publications

Posters: cADDis cAMP Assay 

Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling

PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells

GPCR Biology

Increase your understanding of drug effects and GPCR biology with bright fluorescent assays for Gs, Gi, and Gq signaling in living cells.

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