cADDis: Live Cell cAMP Assay Kit

Detect Gs or Gi signaling with cADDis

  • Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor. • Plate reader or imaging system compatible

Screening in disease relevant cells

Genetically encoded sensor packaged in a variety of vectors for robust expression in any cell type.

Multiplex Assays

Combine red and green sensors to measure multiple GPCR pathways simultaneously

A robust and versatile cAMP assay kit:

 

cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.

  • Screen Gs or Gi-coupled GPCRs in living cells
  • Viral vector delivery to practically any cell type
  • Target to microdomains or specific cells in mixed cultures

cADDis cAMP assay performance:

 

Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficiently deliver to most cell types. Purified BacMam, AAV, Lenti vectors by request.

  • Easily detectable on plate readers or imaging systems.
  • Controllable expression in BacMam vector.
  • High signal to noise ratio

Simple protocol:

 

Provided online is a simple cAMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.

cADDis – cAMP Assay Application Notes