cADDis: Live Cell cAMP Assays
-
#D0200G Green Down cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200G Green Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#X0200G Green Gi cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200R Red Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0205G Green Upward cADDis cAMP (Promoter CAG)
$695.00 – $2,395.00
Detect Gs or Gi signaling with cADDis, our cAMP Assay
- Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor
- Plate reader or imaging system compatible

cADDis cAMP Assay Performance
Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Easily detectable on plate readers or imaging systems
- Controllable expression in BacMam vector
- High signal-to-noise ratio
Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.
Multiplex Assays
- Combine red and green sensors to measure multiple GPCR pathways simultaneously
A Robust and Versatile cAMP Assay Kit:
cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.
- Screen Gs or Gi-coupled GPCRs in living cells
- Robust expression in practically any cell type
- Target to microdomains or specific cells in mixed cultures
Simple protocol:
Provided online is a simple cAMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.
- Text and video protocols online
- No lysis or complex liquid handling
- Single channel, non-FRET signal
Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.
It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.
Recent Publications
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. Science Advances. September 2022. (bioRxiv)
- E. Blythe, M. von Zastrow. A discrete mode of endosomal GPCR signaling that does not require β-arrestins. bioRxiv. September 2022.
- ER McGlone, et al. Hepatocyte cholesterol content modulates glucagon receptor signaling. Molecular Metabolism. September 2022.
- B. Barsi-Rhyne, et al. Discrete GPCR-triggered endocytic modes enable β-arrestins to flexible regulate cell signaling. bioRxiv. July 2022.
- J. Xu, J. Pluznick. Key amino acids alter activity and trafficking of a well-conserved olfactory receptor. Cell Physiology. June 2022.
- C. Zhang, et al. A brainstem circuit for nausea suppression. Cell Reports. June 2022.
- J. Hansen, et al. A cAMP signalome in primary cilia drives gene expression and kidney cyst formation. EMBO Reports. June 2022.
- S. Ansari, et al. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv. May 2022.
- E. Porpiglia, et al. Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration. bioRxiv. April 2022.
- S. Bitsi, et al. Divergent acute versus prolonged in vivo GLP-1R responses in β-arrestin 2-deleted primary beta cells. bioRxiv. April 2022.
- Y. Mizobuchi, et al. Ketamine Improves Desensitization of μ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine. biomolecules. March 2022.
- B. Polacco, et al. Profiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors. bioRxiv. March 2022.
- D. Lovinger, et al. Local modulation by presynaptic receptors controls neuronal communication and behavior. Nature Reviews Neuroscience. February 2022.
- F. De Logu, et al. Schwann cell endosome CGRP signals elicit peri orbital mechanical allodynia in mice. Nature Communications. February 2022.
- A. Lutas, et al. History-dependent dopamine release increases cAMP levels in most basal amygdala glutamatergic neurons to control learning. Cell Reports. January 2022.
- G. Sancar, et al. FGF1 and insulin control lipolysis by convergent pathways. Cell Metabolism. January 2022.
- S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. bioRxiv. December 2021.
- Y. Karasawa, et al. In Vitro Analyses of Spinach-Derived Opioid Peptides, Rubiscolins: Receptor Selectivity and Intracellular Activities through G-protein- and β-arrestin-Mediated Pathways. Molecules. October 2021.
- A. White, et al. Spatial Bias in cAMP generation determines biological responses to PTH type 1 receptor activation. Science Signaling. October 2021.
- J. Janetzko, et al. Membrane phosphoinositides stabilize GPCR-arrestin complexes and offer temporal control of complex assembly and dynamics. bioRxiv. October 2021.
- S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
cADDis – cAMP Assay Posters
Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
Order Fluorescent cADDis cAMP Kits:
-
#D0200G Green Down cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200G Green Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#X0200G Green Gi cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200R Red Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0205G Green Upward cADDis cAMP (Promoter CAG)
$695.00 – $2,395.00