cADDis: Live Cell cAMP Assays
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#D0200G Green Down cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200G Green Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#X0200G Green Gi cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200R Red Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0205G Green Upward cADDis cAMP (Promoter CAG)
$695.00 – $2,395.00
Detect Gs or Gi signaling with cADDis, our cAMP Assay
- Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor
- Plate reader or imaging system compatible
Downward cADDis starts out bright and decreases in fluorescence when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers and helps with imaging cells that have low expression efficiency.
Upward cADDis starts out dim and increases in fluorescence when when cAMP levels increase. This cAMP assay is easily detected on fluorescence plate readers or imaging systems.
cADDis cAMP Assay Performance
Off the shelf kits include the cAMP sensor in BacMam, a BSL-1 viral vector for efficient delivery to most cell types. Purified BacMam, AAV, Lenti vectors by request.
- Easily detectable on plate readers or imaging systems
- Controllable expression in BacMam vector
- High signal-to-noise ratio
Red DAG sensor multiplexed with a green cAMP sensor indicates dose-dependent signaling via a calcitonin receptor.
Multiplex Assays
- Combine red and green sensors to measure multiple GPCR pathways simultaneously
A Robust and Versatile cAMP Assay Kit:
cADDis is a genetically-encoded fluorescent cAMP assay that detects changes in cAMP in real time.
- Screen Gs or Gi-coupled GPCRs in living cells
- Robust expression in practically any cell type
- Target to microdomains or specific cells in mixed cultures
Simple protocol:
Provided online is a simple cAMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.
- Text and video protocols online
- No lysis or complex liquid handling
- Single channel, non-FRET signal
Check out this Live Cell Imaging video from BioTek Instruments: Kinetic Characterization of Gs- and Gi-dependent regulation of cAMP.
It’s a brief animated presentation demonstrating expression & kinetic monitoring of our fluorescent cADDis cAMP Assay in live cells.
Recent Publications
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. Science Advances. September 2022. (bioRxiv)
- E. Blythe, M. von Zastrow. A discrete mode of endosomal GPCR signaling that does not require β-arrestins. bioRxiv. September 2022.
- ER McGlone, et al. Hepatocyte cholesterol content modulates glucagon receptor signaling. Molecular Metabolism. September 2022.
- B. Barsi-Rhyne, et al. Discrete GPCR-triggered endocytic modes enable β-arrestins to flexible regulate cell signaling. bioRxiv. July 2022.
- J. Xu, J. Pluznick. Key amino acids alter activity and trafficking of a well-conserved olfactory receptor. Cell Physiology. June 2022.
- C. Zhang, et al. A brainstem circuit for nausea suppression. Cell Reports. June 2022.
- J. Hansen, et al. A cAMP signalome in primary cilia drives gene expression and kidney cyst formation. EMBO Reports. June 2022.
- S. Ansari, et al. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv. May 2022.
- E. Porpiglia, et al. Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration. bioRxiv. April 2022.
- S. Bitsi, et al. Divergent acute versus prolonged in vivo GLP-1R responses in β-arrestin 2-deleted primary beta cells. bioRxiv. April 2022.
- Y. Mizobuchi, et al. Ketamine Improves Desensitization of μ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine. biomolecules. March 2022.
- B. Polacco, et al. Profiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors. bioRxiv. March 2022.
- D. Lovinger, et al. Local modulation by presynaptic receptors controls neuronal communication and behavior. Nature Reviews Neuroscience. February 2022.
- F. De Logu, et al. Schwann cell endosome CGRP signals elicit peri orbital mechanical allodynia in mice. Nature Communications. February 2022.
- A. Lutas, et al. History-dependent dopamine release increases cAMP levels in most basal amygdala glutamatergic neurons to control learning. Cell Reports. January 2022.
- G. Sancar, et al. FGF1 and insulin control lipolysis by convergent pathways. Cell Metabolism. January 2022.
- S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. bioRxiv. December 2021.
- Y. Karasawa, et al. In Vitro Analyses of Spinach-Derived Opioid Peptides, Rubiscolins: Receptor Selectivity and Intracellular Activities through G-protein- and β-arrestin-Mediated Pathways. Molecules. October 2021.
- A. White, et al. Spatial Bias in cAMP generation determines biological responses to PTH type 1 receptor activation. Science Signaling. October 2021.
- J. Janetzko, et al. Membrane phosphoinositides stabilize GPCR-arrestin complexes and offer temporal control of complex assembly and dynamics. bioRxiv. October 2021.
- S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
cADDis – cAMP Assay Posters
Measuring dynamic and real-time cAMP levels using cADDis, a live-cell indicator for Gs and Gi signaling
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
Order Fluorescent cADDis cAMP Kits:
-
#D0200G Green Down cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200G Green Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#X0200G Green Gi cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0200R Red Up cADDis cAMP Assay Kit
$695.00 – $2,395.00 -
#U0205G Green Upward cADDis cAMP (Promoter CAG)
$695.00 – $2,395.00
