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#U0261G AKAP targeted cADDis cAMP Sensor – AKAP18a

Coming soon, please contact us for additional details.

AKAP18a-targeted cADDis cAMP sensor. Genetically-encoded and packaged in Montana Molecular’s Big Sky BacMam vector for easy transduction into a wide variety of cell types.


 

This product is currently out of stock and unavailable.

U0261G is a fusion of the complete AKAP18A protein and the cADDis biosensor. In HEK 293 cells, the AKAP18a-targeted cADDis sensor appears to be localized to the plasma membrane and intracellular membranes.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP18a-targeted cADDis cAMP sensor. An increase in fluorescence indicates increase in cAMP. cADDis is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.

 

Materials in the Kit:

• Green AKAP18a-targeted cADDis cAMP sensor BacMam
• Sodium Butyrate, 500 mM in H2O
• β2 Adrenergic Receptor BacMam
• Isoproterenol 10 mM, in 10mM HCl


AKAP18a  (Also known as AKAP7)

AKAP 18a binds with the RII subunit of PKA and anchors it to the plasma membrane. 18a interacts with L-type calcium channels and has been implicated in mediating GLP-1 signaling in pancreatic beta-islet cells (Doyle & Egan, 2007; Fraser et al., 1998)

Cell lines

In polarized endothelial cells, it is directed to the lateral surface of the plasma membrane (Trotter et al., 1999).  In HEK 293 cells, the targeted cADDis sensor appears to be localized to the plasma membrane and intracellular membranes.   Co-transfection experiments show that this AKAP can interact with L-type calcium channels (Fraser et al., 1998)

Cardiovascular system

AKAP18a is involved in excitation-contraction coupling in the heart (Ercu & Klussmann, 2018).

Additional information

Protocol for Use

coming soon

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

MSDS

https://montanamolecular.com/msds-for-caddis-assays/

More Info

https://montanamolecular.com/targeted-caddis-camp-assays/

Publications

https://montanamolecular.com/scientific-publications/

Kit Volume

5mL

Terms of Sale

https://montanamolecular.com/terms-of-sale/

GPCR Assay References
cADDis cAMP Assay References
DAG Assay References