Membrane Targeted AKAP18a cADDis cAMP Assay (U0261G)

U0261G is a fusion of the complete AKAP18A/AKAP7 protein and the cADDis biosensor. In HEK 293 cells, the AKAP18a-targeted cADDis sensor appears to be localized to the plasma membrane and intracellular membranes.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP18a-targeted cADDis cAMP sensor. An increase in fluorescence indicates increase in cAMP. cADDis is genetically-encoded and packaged in Montana Molecular's Big Sky BacMam vector for consistent, titratable transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day.

Materials in the Kit:

• Green AKAP18a/AKAP7-targeted cADDis cAMP sensor BacMam
• Sodium Butyrate, 500 mM in H₂O
• β2 Adrenergic Receptor BacMam with nuclear red label
• Isoproterenol 10 mM, in 10mM HCl

AKAP18a (Also known as AKAP7)

AKAP18a binds the RII subunit of PKA and anchors it to the plasma membrane. AKAP18a interacts with L-type calcium channels and has been implicated in mediating GLP-1 signaling in pancreatic beta-islet cells (Doyle & Egan, 2007; Fraser et al., 1998).

In the cardiovascular system, AKAP18a is involved in excitation-contraction coupling in the heart (Ercu & Klussmann, 2018) and in polarized endothelial cells, it is directed to the lateral surface of the plasma membrane (Trotter et al., 1999).