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#U0262G AKAP Targeted cADDis cAMP Sensor – AKAP18b (Prototype)

Prototype sensor, please contact us before ordering.

AKAP18b-targeted cADDis cAMP sensor. Genetically-encoded and packaged in Montana Molecular’s Big Sky BacMam vector for easy transduction into a wide variety of cell types.


 

This product is currently out of stock and unavailable.

Prototype sensor, please contact us before ordering.

U0262G is a fusion of the complete AKAP18b protein to the cADDis biosensor. In HEK293T cells the fluorescence is targeted to the plasma membrane.  This sensor responds nicely to isoproterenol stimulation of endogenous β adrenergic receptors of HEK293 cells.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP18b-targeted cADDis cAMP sensor. Increase in fluorescence indicates increase in cAMP presence. cADDis is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.

Materials in the Kit:

• Green AKAP18b-targeted cADDis cAMP sensor BacMam
• Sodium Butyrate, 500 mM in H2O
• β2 Adrenergic Receptor BacMam
• Isoproterenol 10 mM, in 10mM HCl


AKAP18b (Also known as AKAP7)

AKAP 18b is identical in sequence to AKAP 18a except that it contains an additional 23 amino acid insert that directs it to the apical surface in polarized endothelial cells. (Trotter et al., 1999).  The complete AKAP18b protein is fused to the cADDis biosensor.

Cell lines

Fused to the cADDis sensor, the fluorescence is targeted to the plasma membrane of HEK 293 cells.  This sensor, expressed in HEK 293 cells, responds nicely to isoproterenol stimulation of endogenous β adrenergic receptors.

Additional information

Protocol for Use

coming soon

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

MSDS

https://montanamolecular.com/msds-for-caddis-assays/

More Info

https://montanamolecular.com/targeted-caddis-camp-assays/

Publications

https://montanamolecular.com/scientific-publications/

Kit Volume

5mL

Terms of Sale

https://montanamolecular.com/terms-of-sale/

GPCR Assay References
cADDis cAMP Assay References
DAG Assay References