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Diabetes

Gain insight into diabetes disease mechanisms and GLP-1 receptor pharmacology with bright fluorescent biosensor assays.

Examine Biased agonism at the GLP-1 receptor in cell models and GPCR second messenger signaling in pancreatic islet cells.

 

GPCR Signaling in Diabetes

Monitor GPCR second messenger signaling kinetics with live-cell, fluorescent GPCR assays. Green and red fluorescent biosensor assays can be combined to detect simultaneous signaling in the same cells or wells. Co-transduce GPCRs like the GLP-1 receptor (#Z0600N) in cell models of diabetes. Monitor bright fluorescent assay signals on automated plate readers or imaging systems.

 

DAG Assay

 PIP2 Assay

cADDis cAMP Assay

GECO Calcium Assay

Dose-dependent cAMP responses in HEK293 cells co-expressing GLP-1 receptor.

Expression in Pancreatic Islet Cells

Use assay-ready BacMam vectors to express fluorescent biosensors in pancreatic islets and image signaling kinetics. Target expression to specific cell sub-populations with custom promoters.

Read about research utilizing our assays in pancreatic islet cells:

Mouse Pancreatic Islets

Human Pancreatic Islets

Mouse α-Cells

Alternate viral vectors (AV, Lentivirus, AAV) are available by special order as well.

 

BacMam-mediated cADDis cAMP Sensor expression in a pancreatic islet. Shigeto, et al. 2016.

GLP1-R Biased Agonism

Fluorescent Green β-arrestin-GLP-1 kits are now available! Use cADDis cAMP, DAG, and arrestin assays to examine biased agonism at the GLP-1 receptor in cell models.

β-Arrestin GLP-1 kits include the Green β-arrestin sensor, GLP-1R, and GRK 5 packaged in BacMam.

 

 

 

 

 

 

 

GLP-1 Exendin-4 Kinetic dose-response data collected by the Green Arrestin sensor in HEK 293T cells.

Contact Us

If you have any questions about using these tools in your cells or would like to request a quote, send us an email at [email protected] We’re happy to help!