Diabetes
Gain insight into diabetes disease mechanisms and GLP-1 receptor pharmacology with bright fluorescent biosensor assays.
Examine Biased agonism at the GLP-1 receptor in cell models and GPCR second messenger signaling in pancreatic islet cells.
GPCR Signaling in Diabetes
Monitor GPCR second messenger signaling kinetics with live-cell, fluorescent GPCR assays. Green and red fluorescent biosensor assays can be combined to detect simultaneous signaling in the same cells or wells. Co-transduce GPCRs like the GLP-1 receptor (#Z0600N) in cell models of diabetes. Monitor bright fluorescent assay signals on automated plate readers or imaging systems.
DAG Assay
PIP2 Assay
cADDis cAMP Assay
GECO Calcium Assay
Dose-dependent cAMP responses in HEK293 cells co-expressing GLP-1 receptor.
Expression in Pancreatic Islet Cells
Use assay-ready BacMam vectors to express fluorescent biosensors in pancreatic islets and image signaling kinetics. Target expression to specific cell sub-populations with custom promoters.
Read about research utilizing our assays in pancreatic islet cells:
Mouse Pancreatic Islets
- S. Bitsi, et al. Divergent acute versus prolonged in vivo GLP-1R responses in β-arrestin 2-deleted primary beta cells. bioRxiv. April 2022.
- A. Kim, et al. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes. eLife. November 2021. (bioRxiv)
- M. Draper, et al. Imaging Meets Cytometry: Analyzing Heterogenous Functional Microscopic Data from Living Cell Populations. J. Imaging. January 2021.
- N. Daram, et al. Glucagon-like Peptide 1 Shows Glucose Dependence in Regulating Islet Hormone Secretion. Metabolism. March 2020.
- M. Marasco et al. Interleukin-6 Reduces b-Cell Oxidative Stress by Linking Autophagy With the Antioxidant Response. Diabetes. August 2018.
- A. Hamilton et al. Adrenaline Stimulates Glucagon Secretion by Tpc2-Dependent Ca2+ Mobilization From Acidic Stores in Pancreatic α-Cells. Diabetes. June 2018.
- M. Shigeto, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clinical Invest. 2015.
Human Pancreatic Islets
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. bioRxiv. December 2021.
- J. Almaça, et al. Human beta cells produce and release serotonin to inhibit glucagon secretion from alpha cells.Cell Reports, Volume 17, Issue 12, 2016.
Mouse α-Cells
- J. Knudsen, et al. Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production. Cell Metabolism. February 2019.
Alternate viral vectors (AV, Lentivirus, AAV) are available by special order as well.
BacMam-mediated cADDis cAMP Sensor expression in a pancreatic islet. Shigeto, et al. 2016.
GLP1-R Biased Agonism
Fluorescent Green β-arrestin-GLP-1 kits are now available! Use cADDis cAMP, DAG, and arrestin assays to examine biased agonism at the GLP-1 receptor in cell models.
β-Arrestin GLP-1 kits include the Green β-arrestin sensor, GLP-1R, and GRK 5 packaged in BacMam.
GLP-1 Exendin-4 Kinetic dose-response data collected by the Green Arrestin sensor in HEK 293T cells.
Contact Us
If you have any questions about using these tools in your cells or would like to request a quote, send us an email at info@montanamolecular.com. We’re happy to help!
