Live Cell Paints
Montana Molecular’s live cell painting tools – Paints – enable visualization of organelles, subcellular structures, and cell morphology in the context of drug discovery and disease research. Use the bright, fluorescent tools to investigate drug effects, monitor metabolic activities, and identify disease-associated phenotypes.
- Packaged in a BacMam viral vector for consistent, titratable expression in a wide range of cell types – including many primary and iPSC cultures
- Easily co-transduce red and green fluorescent Paints, biosensors, and other BacMam-packaged constructs
- Capture multiple timepoints in a single well
- Label intracellular structures for phenotypic screens
- No fixation, staining, or cell lysis required
Green TauPaint expresses the bright mNeon Green fluorescent protein fused to the Tau protein.
Explore our catalog of organelle labels below:
mNeon Green or Montana Paintbrush Red fluorescent proteins targeted to the mitochondria using the Cox8 motif.
Green MitoPaint (#P1010G) and Red Lamp2Paint (#P1030R) Expressed in HEK293s
Red NLSPaint (#P1020R) expresses the Montana Paintbrush Red fluorescent protein targeted to the nucleus using an NLS targeting motif.
Green H2BPaint (#P1020G) expresses the mNeon Green fluorescent protein, targeted to the nucleus using Histone 2B (H2B).
Red NLSPaint (#P1020R) Expressed in HEK293s along with Green Lamp2Paint (#P1030G)
Green H2BPaint (#P1020G) Expressed in HEK293s along with Red Lamp2Paint (#P1030R)
Lysosomal Labels (Lamp2Paint)
mNeon Green or Montana Paintbrush Red fluorescent proteins targeted to the lysosome using Lamp2. Red Lamp2Paint (#P1030R) is shown below, Green Lamp2Paint (#P1030G) is also available.
Red Lamp2Paint (#P1030R) Expressed in iPSC-derived neurons
Golgi Labels (GiantinPaint)
Both bright red or green fluorescent proteins targeted to the Golgi using Giantin are available.
Green GiantinPaint (#P1040G) expressed in HEK293 cells along with Red NLSPaint (#P1020R).
Cytoskeletal Labels
Label cytoskeletal structures with mNeon Green and Montana Paintbrush Red fluorescent proteins fused to Tau, human Beta Actin, and Alpha Tubulin.
Green TauPaint (#P1050G) expressed in iPSC-derived neurons. Red TauPaint (#P1050R) is also available.
Red BetaActinPaint (#P1060R) expressed in iPSC-derived neurons with Green H2BPaint. Green BetaActinPaint (#P1060G) is also available.
Green AlphaTubulinPaint (#P1070G) expressed in iPSC-derived neurons. Red AlphaTubulinPaint (#P1070R) is also available.
Endosomal Label (EndoPaint)
EndoPaint is the bright mNeon Green fluorescent protein targeted to the endosome using FYVE.
Green EndoPaint (#P1080G) and Red NLSPaint (#P1020R) co-expressed in HEK293s
Omegasomal Label (OmegaPaint)
OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1).
Green OmegaPaint (#P1090G) expressed in iPSC-derived neurons
Untargeted Fluorescent Proteins
Montana Paintbrush Red fluorescent protein (#P1000R) expressed in HEK293 cells.
mNeon Green fluorescent protein (#P1000G) expressed in HEK293 cells.
Posters
Lighting Up BrainXell iPSC-Derived Neurons with BacMam Delivery of Subcellular Probes in Live Cells
BrainXell iPSC-derived neurons:
- iPSC-derived neurons are increasingly valuable in drug discovery because they can model human-specific genetics and disease phenotypes.
- BacMam vectors are commonly used for rapid gene expression in transformed cell lines, but their optimal use in iPSC-derived neurons is not fully understood.
- We investigated BacMam delivery timing, vector efficacy, and the necessity of HDAC inhibitors in BrainXell iPSC-derived glutamatergic cortical neurons (catalog #BX-0300-30).
We found that:
- Transduction was effective post-seeding at any time over four weeks, and HDAC inhibitors were not necessary.
- Montana Molecular’s BacMam Big Sky vector showed optimal long-term expression and cell health over three weeks in living neurons.
- Cell painting tools showed no mislocalization.
- Montana Molecular’s fluorescent sensors and cell painting tools can be introduced at different iPSC differentiation stages, enabling continuous monitoring of cellular dynamics.
- The biosensors provide for real-time tracking of cAMP, DAG, calcium dynamics, membrane potential, and protein-protein interactions in living neurons.
Montana Molecular Paintbrushes for Live Cell Imaging
Montana Molecular has developed a new suite of fluorescent probes that deliver bright cell painting tools that can be visualized in real-time in living cells producing a wealth of kinetic data to inform drug discovery and disease research.
The Montana Paint Box can:
- Use live cell imaging, no fixation or staining required
- Quantify drug effects on subcellular compartments
- Monitor metabolic activities
- Identify disease-related phenotypes
- Produce robust signal that can be measured using standard fluorescent microscopes and automated plate readers
Order Online or Request a Quote
Use the links below to send us questions, request a quote, purchase kits, or to let us know if there are other fluorescent labels would be helpful in your research. We’re happy to help!
