GPCRs in BacMam
We have a growing catalog of GPCRs packaged in a modified Baculovirus vector, BacMam. BacMam transduction is a low-cost, effective method to express GPCRs in your cells of interest, including in primary and iPSC derived cells. See low cell-to-cell variability in GPCR expression, get consistent results from experiment to experiment, and titrate expression to your desired level. If your receptor is not listed, please see the Custom Projects section below .
GPCR Catalog
Gi Coupled Receptors
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- Human D2 Dopamine Receptor (DRD2)
- Human D3 Dopamine Receptor (DRD3)
- Human M2 Muscarinic Receptor (CHRM2)
- Human A1 Adenosine Receptor (ADORA1)
- Suman Succinate Receptor (SUCNR1)
- Human Kappa Opioid Receptor (OPRK1)
- Human Cannabinoid Receptor Type 1 (CNR1)
- Human Mu Opioid receptor (OPRM1)
- Human Nociceptin Opioid Receptor (OPRX)
- Human Delta Opioid Receptor (OPRD1)
- Human C-X-C Chemokine Receptor Type 4 (CXCR4)
- Human Lysophosphatidic Acid 1 Receptor (LPAR1)
- Human Serotonin 5-HT1A Receptor (HTR1A)
- Human Cannabinoid Receptor Type 2 (CNR2)
- Human Serotonin 5-HT1B Receptor (HTR1B)
- Human Somatostatin Receptor Type 2 (SSTR2)
Gs Coupled Receptors
- Human β2 Adrenergic Receptor (ADRB2)
- Human D1 Dopamine Receptor (DRD1)
- Human Calcitonin Receptor (CALCR)
- Human GLP-1 Receptor (GLP1R)
- Human Vasopressin Receptor (AVPR2)
- Human Parathyroid Hormone 1 Receptor (PTH1R)
- Human Gastric Inhibitory Polypeptide Receptor (GIPR)
- Human Glucagon Receptor (GCGR)
Gq Coupled Receptors
- Human Calcitonin Receptor (CALCR)
- Human Oxytocin Receptor (OXTR)
- Human Angiotensin II Type I Receptor (AGTR1)
- Human M3 Muscarinic Receptor (CHRM3)
- Human Serotonin 5HT2A Receptor (HTR2A)
- Human Serotonin 5HT2C Receptor (HTR2C)
- Human Lysophosphatidic Acid 1 Receptor (LPAR1)
- Human Proteinase-Activated 2 Receptor (PAR2-F2RL1)
- Human M1 Muscarinic Receptor (CHRM1)
*These contain a constitutively expressed, nuclear targeted, red or green fluorescent protein. This fluorescent protein is a bicistronic transcript and does not alter the sequence of the GPCR.
Simple Protocol
- No cell lysis
- BSL-1 Vector
- Minimal Liquid Handling
- Low Toxicity
- Benefits of Using BacMam
Custom Projects
Your GPCR of interest isn’t listed? Or you have a specific modification in mind? We are more than happy to discuss your project with you. Send an email to info@montanamolecular.com.
Some common additions to receptors include:
- Constitutively expressed fluorescent proteins or fluorescent tags on GPCRs
- Genetic targeting to microdomains within the cell (pictured: Plasma Membrane-targeted cADDis cAMP Sensor)
- Cell-specific promoters or Cre-inducible systems to target cellular subpopulations
Pair with a GPCR Signaling Assay
To look at receptor signaling, our GPCR second messenger sensors for cAMP, DAG, PIP2, Calcium, and arrestin can be co-transduced along with your receptor. More information on our Gs, Gi, and Gq signaling assays can be found on our GPCR Biology page.
