Phosphodiesterase Biology

Cyclic GMP is an important second messenger in many cellular processes, including phototransduction, smooth muscle relaxation, and synaptic plasticity. GENIe is a genetically-encoded fluorescent assay that detects cGMP signaling in living cells. The assay can be used to determine the specificity of PDE inhibitors. GENIe can be combined with different colored sensors to measure multiple signals simultaneously. The fluorescence decreases in response to increasing levels of cGMP, and can be readily detected on fluorescence microscopes and automated fluorescence plate readers.

Our green fluorescent cyclic GMP sensor, GENIe, in HEK293 cells responding to Sodium Nitroprusside (SNP).

 

GENIe, a fluorescent assay for cGMP

  • Study cGMP production or PDE inhibition
  • Measure kinetic response in living cells.
  • Simultaneously detect cAMP with red cADDis
  • Available in a BacMam vector for efficient delivery to primary and iPSC cultures.
  • Monitor fluorescence changes on automated plate readers or imaging systems.

Monitor cGMP production or phosphodiesterase inhibition in living cells