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#D2000G Borealis-V2R


Borealis is a fluorescent biosensor assay for arrestin recruitment. This kit captures arrestin recruitment kinetics at the unmodified Vasopressin Type 2 Receptor (V2R) in live cells.

A kinetic biosensor assay for arrestin recruitment converts conformational changes in arrestin-3 (β-arrestin-2) to changes in fluorescence intensity. This kit detects arrestin recruitment at the unmodified Vasopressin Type 2 Receptor (V2R). The Borealis Arrestin Sensor, and V2R, are packaged in a BacMam viral vector for easy delivery to most cell types. Validated for use on fluorescence imaging systems and automated fluorescence plate readers.

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Materials in the Kit:

• Green Borealis Biosensor for arrestin, in BacMam

• Unmodified Vasopressin Type 2 Receptor (V2R), in BacMam

• Sodium Butyrate, 500mM in H2O

• Control Receptor: Angiotensin II-Type 1 (AT1R), in BacMam (provided as a positive control)

• Control Agonist: Angiotensin II, 5mM in H2O (provided as a positive control)

Additional information

Protocol for Use


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.


Scientific Publications

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Kit Volume

10mL, 30mL, 60mL

GPCR Assay References:
cADDis cAMP Assay References
DAG Assay References