BacMam Transducible Cells

BacMam efficiently transduces most mammalian cells, including primary cultures, cell lines, and iPSC derived cells. BacMam transduction provides much more consistent cell-to-cell expression compared to plasmid transfection, and researchers have used BacMam to express our sensors in primary neurons, neonatal cardiomyocytes, human and rodent pancreatic islets, and more! This is a non-exhaustive list of cells that have been shown to be transducible by BacMam.

Pancreas

Primate:

Pancreatic b-cells | Ma, L. et al. Diabetes 49, 1986–1991 (2000).

Human beta cells | Almaça, J., et al. Cell Reports. Vol. 17, Issue 12 (2016)

Primary human pancreatic islet cells | Ma, L. et al. Diabetes 49, 1986–1991 (2000).

Rodent:

Primary pancreatic islet cells | Ma, L. et al. Diabetes 49, 1986–1991 (2000).

Brain

Primate:

PSC- derived Neural Stem Cells | Efthymiou, A. et al. J Biomol Screen 19, 32-43 (2014).

CHP212 (neuroblastoma) | Sarkis, C. et al. Proc Natl Acad Sci U S A 97, 14638–14643 (2000).

Glioma: BTL2C6, H52, H80, MGC, U373, | Lackner, A. et al. Analytical Biochemistry 380, 146–148 (2008).

Glioma: BT4C | Kaikkonen, M.U. et al. Gene Ther 13, 304–312 (2005).

Glioma: BT325, H4, SW1088, SW1783, U87, U251 | Wang, C.-Y. et al. Cancer Res 66, 5798–5806 (2006).

Glioma: U373MG | Kronschnabl, M. & Stamminger, T. J. Gen. Virol. 84, 61–73 (2003).

Human embryonic neural stem cells | Zeng, J. et al. Mol Ther 17, 1585–1593 (2009).

IMR-32 neuroblastoma (ATCC CCL-127) following differentiation | Shoji, I. et al. J. Gen. Virol. 78, 2657–2664 (1997).

Primary human astrocytes | Sarkis, C. et al. Proc Natl Acad Sci U S A 97, 14638–14643 (2000).

Primary human glial cells | Sarkis, C. et al. Proc Natl Acad Sci U S A 97, 14638–14643 (2000).

Primary human neuroepithelial and neuroblastic cells | Sarkis, C. et al. Proc Natl Acad Sci U S A 97, 14638–14643 (2000).

SHSY-5Y (neuroblastoma) | Andersson, M. et al. BMC Cell Biology 8, 6 (2007).

Rodent:

C17.2 cells (differentiated, multi potent neural stem cell line | Li, Y., Yang, Y. & Wang, S. Experimental Physiology 90, 39–44 (2005).

Dorsal root ganglion neurons | Liu, L., et al. bioRxiv doi.org/10.1101/410878 (2018).

Neuroblastoma (N2a) | Sarkis, C. et al. Proc Natl Acad Sci U S A 97, 14638–14643 (2000).

Brain choroid plexus cells (in vivo) | Laitinen, O.H. et al. Nucleic Acids Research 33, e42–e42 (2005).

PC12 | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

Primary cerbellar granule neurons | Li, Y., Yang, Y. & Wang, S. Experimental Physiology 90, 39–44 (2005).

Primary glial cells (astrocytes) | Wang, C.Y. & Wang, S. Gene Ther 13, 1447–1456 (2006).

Primary spiral ganglion neurons | Wang, J. et al. NeuroReport 18, 1329–1333 (2007).

Heart

Primate:

Primary human cardiomyocytes | Grassi, G. et al. Arch Virol 151, 255–271 (2005).

Primary human coronary artery endothelia cells (HCEC) | Grassi, G. et al. Arch Virol 151, 255–271 (2005).

Primary human coronary smooth muscle cells (HCASMC) | Grassi, G. et al. Arch Virol 151, 255–271 (2005).

Primary aortic smooth muscle cells (RaASMC) | Mähönen, A.J. et al. J. Biotechnol. 131, 1–8 (2007).

Prenatal cardiomyocytes (hCM) | Grassi, G. et al. Arch Virol 151, 255–271 (2005).

Rodent:

Primary aortic smooth muscle cells (RaASMC) | Mähönen, A.J. et al. J. Biotechnol. 131, 1–8 (2007).

Other Mammalian:

Porcine coronary artery smooth muscle cells (pCSMC) | Grassi, G. et al. Arch Virol 151, 255–271 (2005).

Bone

Lung

Primate:

HASM (human airway smooth muscle) | Johnson, T.B. et al. American Journal of Respiratory Cell and Molecular Biology. Vol 58, No. 4 (2018)

DMS 114 (ATCC CRL-2066, small cell lung carcinoma) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

Embryonic lung fibroblasts | Lopez, P., Van Sant, C. & Roizman, B. Journal of Virology 75, 3832–3840 (2001).

MRC-5 (lung fibroblast) | Palombo, F. et al. J. Virol. 72, 5025–5034 (1998).

WI38 (human lung fibroblast) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

Kidney

Primate:

CV-1 (normal African green monkey kidney fibroblast cells) | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

Rodent:

BHK (kidney cell line) | Liang, C.Y. et al. Arch Virol 149, 51–60 (2003).

BHK | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

Primary kidney cells (MKC) | Liang, C.Y. et al. Arch Virol 149, 51–60 (2003).

Other Mammalian:

CPK (porcine kidney) | Shoji, I. et al. J. Gen. Virol. 78, 2657–2664 (1997).

FS-L3 (porcine kidney) epithelial cell line | Shoji, I. et al. J. Gen. Virol. 78, 2657–2664 (1997).

PK-15 (porcine kidney; ATCC CCL-33 | Liang, C.Y. et al. Arch Virol 149, 51–60 (2003).

LLC-PK1 (kidney proximal tubule) cell line | Hassan, N.J. et al. Prot. Expr. Purif. 47, 591–598 (2006).

Immune System

Primate:

Primary dendritic cells | Strauss, R. et al. Mol Ther 15, 193–202 (2007).

Rodent:

P388D1 (ATCC CCL-46, lymphoma) | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

Cell Lines

Other

Primate:

143TK-(fibroblast) | Ye, G.-J., Vaughan, K.T., Vallee, R.B. & Roizman, B. J. Virol. 74, 1355–1363 (2000).

BGC-223 (breast carcinoma) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

EA.hy926 (hybridoma of HUVEC and A549) | Mähönen, A.J. et al. J. Biotechnol. 131, 1–8 (2007).

Human mesenchymal stem cells (MSC) (from umbilical cord blood, bone marrow) | Ho, Y.-C. et al. J. Gene Medicine 7, 860–868 (2005).

K-562 (ATCC CCL-243, chronic myelogenous leukemia) | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

KATO-III (HTB-103, gastric carcinoma) | Shoji, I. et al. J. Gen. Virol. 78, 2657–2664 (1997).

MCF-7 (Gastric carcinoma) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

MT-2 | Shoji, I. et al. J. Gen. Virol. 78, 2657–2664 (1997).

Primary human fibroblasts (HFB) | Grassi, G. et al. Arch Virol 151, 255–271 (2005).

Primary human keratinocytes (HEK) | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

Primary umbilical vein endothelial cells (HUVEC) | Kronschnabl, M. & Stamminger, T. J. Gen. Virol. 84, 61–73 (2003).

SK-N-MC | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

SK-OV-3 (ATCC HTB-77, adenocarcinoma) | Kaikkonen, M.U. et al. Gene Ther 13, 304–312 (2005).

W12 (human cervical keratinocyte) | Condreay, J.P. et al. Proc Natl Acad Sci U S A 96, 127–132 (1999).

Rodent:

3T3 (mouse embryo fibroblasts) (NIH3T3) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

C2C12 (myoblast) | Shen, H.C. et al. The Journal of Gene Medicine 10, 1190–1197 (2008).

JC (adenocarcinoma) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

L-929 (ATCC CCL-1, subcutaneous connective tissue) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

Primary chondrocytes | Shen, H.C. et al. The Journal of Gene Medicine 9, 470–478 (2007).

Primary chondrocytes (intervertebral disc nucleus pulposus cells, in vitro and in vivo) | Liu, X. et al. Spine 31, 732–735 (2006).

Primary myoblasts | Shen, H.C. et al. The Journal of Gene Medicine 10, 1190–1197 (2008).

PT67 (embryo fibroblast) | Cheng, T. et al. World J Gastroenterol 10, 1612–1618 (2004).

RGM I | Shoji, I. et al. J. Gen. Virol. 78, 2657–2664 (1997).

Sol 8 (myoblast) | Shen, H.C. et al. The Journal of Gene Medicine 10, 1190–1197 (2008).

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