Select Page

#U0263G AKAP Targeted cADDis cAMP Sensor – AKAP1 (Prototype)

Prototype sensor, please contact us before ordering.

AKAP1-targeted cADDis cAMP sensor. Genetically-encoded and packaged in Montana Molecular’s Big Sky BacMam vector for easy transduction into a wide variety of cell types.


 

This product is currently out of stock and unavailable.

Prototype sensor, please contact us before ordering.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP1-targeted cADDis cAMP sensor. Increase in fluorescence indicates increase in cAMP presence. cADDis is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.


Materials in the Kit:

• Green AKAP1-targeted cADDis cAMP sensor BacMam

• Sodium Butyrate, 500 mM in H2O

• β2 Adrenergic Receptor BacMam

• Isoproterenol 10 mM, in 10mM HCl


AKAP1

The gene AKAP1 (also known as AKAP149) encodes several different splice variants that localize differently in the cell.  One of these targets the cADDis cAMP sensor to the mitochondrial outer membrane (Huang et al., 1999).  AKAP1 is a dual AKAP, with the ability to bind both regulatory subunits of PKA. 

AKAP1 plays several intracellular roles.  At the mitochondria, AKAP1 is thought to organize PKA activity and phosphorylation of crucial mitochondrial proteins, including Drp1 (Dickey & Strack, 2011).  There is a variety of evidence that AKAP1 is regulating fundamental mitochondrial biology (Zaccolo et al., 2021). In the cardiac system, AKAP1 is also thought to play a role in the cardiac stress response (Ercu & Klussmann, 2018). AKAP1 also appears to play a role in the protection of neurons (Dagda et al., 2011; Merrill et al., 2011) as well as in morphogenesis (Dickey & Strack, 2011).

Dagda, R. K., Gusdon, A. M., Pien, I., Strack, S., Green, S., Li, C., Van Houten, B., Cherra, S. J., 3rd, & Chu, C. T. (2011). Mitochondrially localized PKA reverses mitochondrial pathology and dysfunction in a cellular model of Parkinson’s disease. Cell Death and Differentiation, 18(12), 1914–1923. https://doi.org/10.1038/cdd.2011.74

Dickey, A. S., & Strack, S. (2011). PKA/AKAP1 and PP2A/Bβ2 regulate neuronal morphogenesis via Drp1 phosphorylation and mitochondrial bioenergetics. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience, 31(44), 15716–15726. https://doi.org/10.1523/JNEUROSCI.3159-11.2011

Ercu, M., & Klussmann, E. (2018). Roles of A-Kinase Anchoring Proteins and Phosphodiesterases in the Cardiovascular System. Journal of Cardiovascular Development and Disease, 5(1). https://doi.org/10.3390/jcdd5010014

Huang, L. J., Wang, L., Ma, Y., Durick, K., Perkins, G., Deerinck, T. J., Ellisman, M. H., & Taylor, S. S. (1999). NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulum. The Journal of Cell Biology, 145(5), 951–959. https://doi.org/10.1083/jcb.145.5.951

Merrill, R. A., Dagda, R. K., Dickey, A. S., Cribbs, J. T., Green, S. H., Usachev, Y. M., & Strack, S. (2011). Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1. PLoS Biology, 9(4), e1000612. https://doi.org/10.1371/journal.pbio.1000612

Zaccolo, M., Zerio, A., & Lobo, M. J. (2021). Subcellular Organization of the cAMP Signaling Pathway. Pharmacological Reviews, 73(1), 278–309. https://doi.org/10.1124/pharmrev.120.000086

Additional information

Protocol For Use

coming soon

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

MSDS

https://montanamolecular.com/msds-for-caddis-assays/

More Info

https://montanamolecular.com/targeted-caddis-camp-assays/

Publications

https://montanamolecular.com/scientific-publications/

Kit Volume

5mL

Terms of Sale

https://montanamolecular.com/terms-of-sale/

GPCR Assay References
cADDis cAMP Assay References
DAG Assay References