#X0200G Green Gi cADDis cAMP Assay Kit

Screen Gi-coupled GPCRs to monitor inhibition of cAMP production on fluorescence plate readers or imaging systems. Standard stock comes with CMV promoter. Other promoters are available, please inquire!


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Description

A simple and robust assay for Gi-coupled receptor signaling without the need for co-factors or cell lysis. cADDis is a genetically-encoded biosensor for cAMP that is packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Measure robust signals 24 hours post transduction on standard fluorescence microscopes and automated plate readers.


Materials in the Kit:

• Green cADDis BacMam

• Sodium Butyrate, 500 mM in H2O

• β2 Adrenergic Receptor BacMam

• Active Gs BacMam

• Isoproterenol 10 mM, in 10mM HCl

 

Product Info & Protocols

Protocol for Use

https://montanamolecular.com/protocols/green-gi-caddis-camp-protocol/

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. Purchases over $600 receive free shipping.

MSDS

https://montanamolecular.com/msds-for-caddis-assays/

Scientific Publications

https://montanamolecular.com/scientific-publications//

More Info

https://montanamolecular.com/live-cell-camp-assay-caddis

Kit Volume

10mL, 30mL, 60mL

Clear

Do you have questions? Contact our Technical Sales Specialist.

Lenti & AAV vectors available by special order

Get a quote here! Lead times vary, please inquire.