#X0200G Green Gi cADDis cAMP Assay Kit
$695.00 – $2,395.00
Screen Gi-coupled GPCRs to monitor inhibition of cAMP production on fluorescence plate readers or imaging systems. Standard stock comes with CMV promoter. Other promoters are available, please inquire!
A simple and robust assay for Gi-coupled receptor signaling without the need for co-factors or cell lysis. cADDis is a genetically-encoded biosensor for cAMP that is packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Measure robust signals 24 hours post transduction on standard fluorescence microscopes and automated plate readers.
Materials in the Kit:
• Green cADDis BacMam
• Sodium Butyrate, 500 mM in H2O
• hD2 Receptor BacMam
• Quinpirole Hydrochloride 2 mM, in sterile H2O
Additional information
| Protocol for Use | https://montanamolecular.com/live-cell-camp-assay-caddis/green-gi-caddis-camp-protocol/ |
|---|---|
| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| MSDS | |
| Scientific Publications | |
| More Info | |
| Kit Volume | 10mL, 30mL, 60mL |
| Terms of Sale |
GPCR Assay References:
Borealis Arrestin Assay References
- H. Schiff, et al. β-arrestin-biased proteinase-activated receptor-2 antagonist C781 limits allergen-induced airway hyperresponsiveness and inflammation. British Journal of Pharmacology. June 2022.
- S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
- S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
- S. Hoare, et al. Analyzing kinetic signaling data for G-protein-coupled receptors. Nature Scientific Reports July 2020.
- S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
cADDis cAMP Assay References
- E. Porpiglia, et al. Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration. Cell Stem Cell. December 2022. (bioRxiv)
- J. Janetzko, et al. Membrane phosphoinositides regulate GPCR-β-arrestin complex assembly and dynamics. Cell. November 2022.
- N. Saito, et al. Gαs-Coupled CGRP Receptor Signaling Axis from the Trigeminal Ganglion Neuron to Odontoblast Negatively Regulates Dentin Mineralization. biomolecules. November 2022.
- J. Xu, et al. An evolutionarily conserved olfactory receptor is required for sex differences in blood pressure. bioRxiv. November 2022.
- B. Barsi-Rhyne, et al. Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling. eLife. October 2022. (bioRxiv)
- S. A. Dai, et al. State-selective modulation of heterotrimeric Gαs signaling with macrocyclic peptides. Cell. September 2022.
- J. H. Cho, et al. Islet primary cilia motility controls insulin secretion. Science Advances. September 2022. (bioRxiv)
- E. Blythe, M. von Zastrow. A discrete mode of endosomal GPCR signaling that does not require β-arrestins. bioRxiv. September 2022.
- ER McGlone, et al. Hepatocyte cholesterol content modulates glucagon receptor signaling. Molecular Metabolism. September 2022.
- J. Xu, J. Pluznick. Key amino acids alter activity and trafficking of a well-conserved olfactory receptor. Cell Physiology. June 2022.
- C. Zhang, et al. A brainstem circuit for nausea suppression. Cell Reports. June 2022.
- J. Hansen, et al. A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. EMBO Reports. June 2022.
- S. Ansari, et al. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv. May 2022.
- S. Bitsi, et al. Divergent acute versus prolonged in vivo GLP-1R responses in β-arrestin 2-deleted primary beta cells. bioRxiv. April 2022.
- Y. Mizobuchi, et al. Ketamine Improves Desensitization of μ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine. biomolecules. March 2022.
- B. Polacco, et al. Profiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors. bioRxiv. March 2022.
- D. Lovinger, et al. Local modulation by presynaptic receptors controls neuronal communication and behavior. Nature Reviews Neuroscience. February 2022.
- F. De Logu, et al. Schwann cell endosome CGRP signals elicit peri orbital mechanical allodynia in mice. Nature Communications. February 2022.
- A. Lutas, et al. History-dependent dopamine release increases cAMP levels in most basal amygdala glutamatergic neurons to control learning. Cell Reports. January 2022.
- G. Sancar, et al. FGF1 and insulin control lipolysis by convergent pathways. Cell Metabolism. January 2022.
- S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
- Y. Karasawa, et al. In Vitro Analyses of Spinach-Derived Opioid Peptides, Rubiscolins: Receptor Selectivity and Intracellular Activities through G-protein- and β-arrestin-Mediated Pathways. Molecules. October 2021.
- A. White, et al. Spatial Bias in cAMP generation determines biological responses to PTH type 1 receptor activation. Science Signaling. October 2021.
- J. Janetzko, et al. Membrane phosphoinositides stabilize GPCR-arrestin complexes and offer temporal control of complex assembly and dynamics. bioRxiv. October 2021.
- Y. Kuroda, et al. Inhibition of endothelin A receptor by a novel, selective receptor antagonist enhances morphine-induced analgesia: Possible functional interaction of dimerized endothelin A and μ-opioid receptors. Biomedicine & Pharmacotherapy. September 2021.
- A. Lutas, et al. Dopamine-dependent cAMP dynamics in basal amygdala glutamatergic neurons. bioRxiv. September 2021.
- F. De Logu, et al. CGRP Signals from Endosomes of Schwann Cells to Elicit Migraine Pain. Research Square. August 2021.
- C. Wu, et al. Discovery of ciliary G protein-coupled receptors regulating pancreatic islet insulin and glucagon secretion. Genes & Development. August 2021. bioRxiv.
- S. Zhang, et al. Hypothalamic dopamine neurons motivate mating through persistent cAMP signalling. Nature. August 2021.
- M. Nuriya, et al. Alkyne-Tagged Dopamines as Versatile Analogue Probes for Dopaminergic System Analysis. Analytical Chemistry. July 2021.
- N. Senese, et al. Antidepressants produce persistent Gαs associated signaling changes in lipid rafts following drug withdrawal. Molecular Pharmacology. May 2021.
- W. Han, et al. Nausea and the Brain: The Chemoreceptor Trigger Zone Enters the Molecular Age. Neuron. February 2021.
- H. Carr, et al. The PDZ Domain Protein SYNJ2BP Regulates GRK-dependent Sst2A Phosphorylation and Downstream MAPK Signaling. Endocrinology. February 2021.
- A. Pronin, et al. Ectopically expressed olfactory receptors OR51E1 and OR51E2 suppress proliferation and promote cell death in a prostate cancer cell line. Journal of Biological Chemistry. February 2021.
- A. Gad, et al. Conserved residues in extracellular loop 2 regulate Stachel-mediated activation of ADGRG2. bioRxiv. February 2021.
- Z. Zhou, et al. Astrocytic cAMP modulates memory via synaptic plasticity. PNAS. January 2021.
- M. Draper, et al. Imaging Meets Cytometry: Analyzing Heterogenous Functional Microscopic Data from Living Cell Populations. J. Imaging. January 2021.
- M. Thomas, et al. Optically activated, customizable, excitable cells. PLOS One. December 2020.
- C. Zhang, et al. Area Postrema Cell Types That Mediate Nausea-Associated Behaviors. Neuron. November 2020.
- T. Patriarchi, et al. An expanded palette of dopamine sensors for multiplex imaging in vivo. Nature Methods. September 2020.
- S. Hoare, et al. Analyzing kinetic signaling data for G-protein-coupled receptors. Nature Scientific Reports July 2020.
- J. Hansen, et al. Nanobody-directed targeting of ontogenetic tools to study signaling in the primary cilium. eLife. June 2020.
- J. Yu et al. N-3 polyunsaturated fatty acids promote astrocyte differentiation and neurotrophin production independent of cAMP in patient-derived neural stem cells. Mol. Psychiatry. June 2020.
- J. Cortes-Troncoso, et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjörgen's Syndrome. JCI Insight. May 2020.
- I. Gapallawa, et al. Bitter taste receptors stimulate phagocytosis in human macrophages through calcium, nitric oxide, and cyclic-GMP signaling Cell. Mol. Life Sci. March, 2020 (bioRxiv)
- N. Daram, et al. Glucagon-like Peptide 1 Shows Glucose Dependence in Regulating Islet Hormone Secretion. Metabolism. March 2020.
- C. Koziol-White et al. Budesonide enhances agonist-induced bronchodilation in human small airways by increasing cAMP production in airway smooth muscle. American Journal of Physiology. February 2020.
- S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
- D. McMahon, et al. Neuropeptide regulation of secretion and inflammation in human airway gland serous cells (bioRxiv)
- R. Forteza, et al. Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects Molecular Biology of the Cell. Dec. 2019
- Nunez, F.J., Johnstone, T.B. et al. Glucocorticoids rapidly activate cAMP production via Gαs to initiate non‐genomic signaling that contributes to one‐third of their canonical genomic effects FASEB Journal, Dec. 2019
- Nunez, F.J., Schulte, N.A., Fogel, D.M., et al. Agonist-specific desensitization of PGE2-stimulated cAMP signaling due to upregulated phosphodiesterase expression in human lung fibroblasts Naunyn-Schmiedeberg’s Arch Pharmacol Dec. 2019
- K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Front. Cell. Neurosci.,December 2019
- MP Pedro et al. Activation of G-Protein Coupled Receptor-Gai Signaling Increases Keratinocyte Proliferation and Reduces Differentiation, Leading to Epidermal Hyperplasia. Journal of Investigative Dermatology. November 2019.
- K. Hilgendorf, et al. Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis Cell November, 2019
- R. Sherpa et al. Sensory primary cilium is a responsive cAMP microdomain in renal epithelia. Scientific Reports. April 2019
- T. Baldwin, et al. Insights into the Regulatory Properties of Human Adenylyl Cyclase Type 9 Molecular Pharmacology. April, 2019
- T. Togo Autocrine purinergic signaling stimulated by cell membrane disruption is involved in both cell membrane repair and adaptive response in MDCK cells Biochem & Biophysical Research Comm. March, 2019
- J. Knudsen, et al. Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production. Cell Metabolism. February 2019.
- C. Ojiaku, et al. Transforming Growth Factor-β1 Decreases β2-Agonist–induced Relaxation in Human Airway Smooth Muscle ATSJournas, Dec 2018
- N. Naim et al. Luminescence-activated nucleotide cyclase regulates spatial and temporal cAMP synthesis. Journal of Biological Chemistry. December 2018.
- Y.A.B. Sierra, et al. Potassium channel-based optogenetic silencing. Nature Communications Nov. 5 2018.
- M. Qadri et al. cAMP attenuates TGF-β’s profibrotic responses in osteoarthritic synoviocytes: involvement of hyaluronan and PRG4. Cell Physiology. September 2018.
- M. Marasco et al. Interleukin-6 Reduces Beta-Cell Oxidative Stress by Linking Autophagy With the Antioxidant Response. Diabetes. August 2018
- X. Chen, et al. Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling. Scientific Reports Aug. 2018.
- P. Tewson, et al. Assay for Detecting Gαi-Mediated Decreases in cAMP in Living Cells. SLAS. July 10, 2018. Request Full Text PDF
- A. Hamilton et al. Adrenaline Stimulates Glucagon Secretion by Tpc2-Dependent Ca2+ Mobilization From Acidic Stores in Pancreatic α-Cells. Diabetes. June 2018.
- T. Buranda, et al. A High-Throughput Flow Cytometry Screen Identifies Molecules that Inhibit Hantavirus Cell Entry. SLAS Discovery. April 2, 2018.
- N. H. Wray, et al. NMDAR-independent, cAMP-dependent antidepressant actions of ketamine. Molecular Psychiatry. April 2, 2018.
- H. Zou, et al. PDE8: A Novel Target in Airway Smooth Muscle. American Journal of Respiratory Cell and Molecular Biology. Vol. 58, No. 4. April 01 2018.
- H. Singh et al. Disruption of lipid-raft localized Gαs/tubulin complexes by antidepressants: a unique feature of HDAC6 inhibitors, SSRI and tricyclic compounds. Neuropsychopharmacology. February 2018.
- T.B. Johnstone, et al. PDE8 is Expressed in Human Airway Smooth Muscle and Selectively Regulates cAMP Signaling by β2AR-AC6. American Journal of Respiratory Cell and Molecular Biology. Dec. 2017.
- T. Togo Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells Biology Open. December 2017.
- K.A. McCrink, et al. β-Arrestin2 Improves Post-Myocardial Infarction Heart Failure via Sarco(endo)plasmic Reticulum Ca2+-ATPase-Dependent Positive Inotropy in Cardiomyocytes. Hypertension. Nov. 2017.
- Q. Wang, et al. Regulator of G protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion. FASEB J. Jul. 7,2017.
- J. King et al. A G protein‐coupled α7 nicotinic receptor regulates signaling and TNF‐α release in microglia. FEBS. July 2017.
- J. Almaça, et al. Human beta cells produce and release serotonin to inhibit glucagon secretion from alpha cells.Cell Reports, Volume 17, Issue 12, 2016.
- P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 11, 2015. Request Full Text PDF
- B.S. Moore, et al. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics. PNAS Nov. 15, 2016.
PIP2 Assay References
- L. Brueggemann, et al. Structural Determinants of Kv7.5 Potassium Channels that Confer Changes in Phosphatidylinositol 4,5-Biphosphate (PIP2) Affinity and Signaling Sensitivity in Smooth Muscle Cells. Molecular Pharmacology. March 2020.
- L.Liu, et al. Gαq sensitizes TRPM8 to inhibition by PI(4,5)P2 depletion upon receptor activation. J.Neurosci. May 2019.
- , and Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor Molecular Pharmacology Nov. 2018
- Y. Ding, et al. Ratiometric biosensors based on dimerization dependent fluorescent protein exchange. Nature Methods, 2015.
- P. Tewson, et al. A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. J. Biomolecular Screening 18, 2013.
DAG Assay References
- G. Sanchez, et al. Coincident Regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid Receptors Opposes Opioid-Mediated Antinociception. Molecular Pharmacology. December 2022.
- Z. Miller, et al. Lidocaine Induces Apoptosis in Head and Neck Squamous Cell Carcinoma Cells Through Activation of Bitter Taste Receptor T2R14. bioRxiv. November 2022.
- M. Doepner, et al. Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling. Science Advances. September 2022.
- A. Kim, et al. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes. eLife. November 2021. (bioRxiv)
- S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
- E. Maguire, et al. The Alzheimer's disease protective P522R variant of PLCG2, consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function. bioRxiv. April 2020.
- L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.
- S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
- A. Kim et al. AVP-induced counter-regulatory glucagon is diminished in type 1 diabetes. BioRxiv. January 2020.
- C Deisl et al. Hypertrophy of human embryonic stem cell–derived cardiomyocytes supported by positive feedback between Ca2+ and diacylglycerol signals. Pflügers Archiv – European Journal of Physiology Jun. 2019
- Herranz, Gonzalo et al. Protein Kinase C δ Regulates the Depletion of Actin at the Immunological Synapse Required for Polarized Exosome Secretion by T Cells. Frontiers in Immunology Apr. 2019
- Ohno, et al. Dynamics of Presynaptic Diacylglycerol in a Sensory Neuron Encode Differences between Past and Current Stimulus Intensity. Cell Reports, Vol. 20, Issue 10. Sept. 2017
- Q. Wang, et al. Regulator of G protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion. FASEB J. Jul. 7,2017.
- Shigeto, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clinical Invest. 2015. doi:10.1172/JCI81975.
- Xingjuan, et al. PKC-dependent Phosphorylation of the H1 Histamine Receptor Modulates TRPC6 Activity. Cells. 2014.
- P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 2015. Request Full Text PDF
- Arttamangkul, et al.Does PKC activation increase the homologous desensitization of μ opioid receptors?British Journal of Pharmacology April 2014
- Tewson, et al. Simultaneous Detection of Ca2+and Diacylglycerol Signaling in Living Cells. PLoS One. 2012.
