#D0400R Red PIP2 Assay Kit

Red fluorescence decreases as PIP2 is hydrolyzed to produce DAG and IP3, activated by GPCRs coupled to Gq. Standard stock comes with CMV promoter. Other promoters are available, please inquire!


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Description

The red PIP2 sensor is based on a dimerization-dependent fluorescent protein. The BacMam vector carrying these sensors is a modified baculovirus, which can be used for delivery to, and expression in, a wide variety of mammalian cell types including primary cultures. Red fluorescence decreases as PIP2 is hydrolyzed to produce DAG and IP3.


Materials in the Kit:

• Red PIP2  Sensor BacMam

• Sodium Butyrate, 500 mM in H2O

• HM1 muscarinic acetylcholine receptor BacMam with nuclear green label in TMN-FH Insect Culture Medium

• Carbachol 25 mM in H2O

 

Product Info & Protocols

Protocol for Use

https://montanamolecular.com/protocols/red-pip2-protocol/

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. Purchases over $600 receive free shipping.

MSDS

https://montanamolecular.com/msds-for-dag-geco-pip2-assays/

Scientific Publications

https://montanamolecular.com/scientific-publications/

Kit Volume

10mL

Clear

Do you have questions? Contact our Technical Sales Specialist.

Lenti & AAV vectors available by special order

Get a quote here! Lead times vary, please inquire.