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#D0300R Red Down DAG Assay Kit


Monitor Gq-mediated changes in diacylglycerol on fluorescence imaging systems with the red fluorescent downward DAG assay. Standard stock comes with CMV promoter. Other promoters are available, please inquire!

Measure diacylglycerol signaling in real time, in the living cells of your choice. This red fluorescent sensor decreases in fluorescence in response to increases in intracellular DAG. Our DAG Assay is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. The red fluorescent downward DAG assay starts bright and decreases in fluorescence in response to increasing DAG. Compatible with use on standard fluorescence microscopes and automated plate readers. Find additional details on the DAG Assays page.

Materials in the Kit:

• Red Down DAG Sensor BacMam

• Sodium Butyrate, 500 mM in H2O

Human M1 muscarinic acetylcholine receptor BacMam with nuclear green label

• Carbachol 25 mM in H2O

Additional information

Protocol for Use


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradablepackaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.


More Info

Scientific Publications

Kit Volume

10mL, 30mL, 60mL

Terms of Sale

GPCR Assay References
cADDis cAMP Assay References
DAG Assay References