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#D0300G Green Down DAG Assay Kit


Screen Gq-coupled GPCRs to monitor increases in DAG on fluorescence plate readers or imaging systems. Standard stock comes with CMV promoter. Other promoters are available, please inquire!

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Measure diacylglycerol signaling in real time, in the living cells of your choice. Our DAG Assay is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add the sensor to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers. This green, fluorescent sensor decreases in fluorescence intensity in response to increases in intracellular DAG. Find additional details on the DAG Assays page.

Materials in the Kit:

• Green Down DAG Sensor BacMam

• Sodium Butyrate, 500 mM in H2O

Human M1 muscarinic acetylcholine receptor BacMam with nuclear red label

• Carbachol 25 mM in H2O

Additional information

Protocol for Use


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

More Info


Scientific Publications

Kit Volume

10mL, 30mL, 60mL

Terms of Sale

GPCR Assay References
cADDis cAMP Assay References
DAG Assay References