Select Page

#D0400G Green PIP2 Assay Kit


Green fluorescence decreases as PIP2 is hydrolyzed to produce DAG and IP3, activated by GPCRs coupled to Gq. Standard stock comes with CMV promoter. Other promoters are available, please inquire!

Featured Publication: 
Structural Determinants of Kv7.5 Potassium Channels That Confer Changes in Phosphatidylinositol 4,5-Bisphosphate (PIP2) Affinity and Signaling Sensitivities in Smooth Muscle Cells

A genetically-encoded biosensor for PIP2 with a dimerization-dependent fluorescent protein. Green fluorescence decreases as PIP2 is hydrolyzed to produce DAG and IP3. The green fluorescent PIP2 assay is genetically encoded and packaged in BacMam providing simple, titratable expression in a variety of cell lines and primary cultures. See the PIP2 Assays page for additional details on this tool.

Materials in the Kit:

• Green PIP2  sensor BacMam

• Sodium Butyrate, 500 mM in H2O

Z0200R human M1 muscarinic acetylcholine receptor BacMam with nuclear red label in TNM-FH Insect Culture Medium

• Carbachol 25 mM in H2O

Additional information

Protocol for Use


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.


More Info

Scientific Publications

Kit Volume

10mL, 30mL, 60mL

GPCR Assay References
cADDis cAMP Assay References
DAG Assay References