#U0901G Green/Red Ratiometric Cell Stress Assay Kit
Bright green fluorescent assay to detect response and reversal of chemical or genetic ER stress response and UPR. CMV promoter.
The cell stress sensor is a genetically-encoded fluorescent biosensor that contains a constitutively expressed red fluorescent protein and produces very bright green fluorescence when the cell endures endoplasmic reticulum (ER-mediated) stress or undergoes the unfolded protein response (UPR). Importantly, both the red and green fluorescent signals are nuclear localized for simple cell segmentation and image analysis. This sensor is based on the XBP1 mRNA which is spliced by the IRE1 protein during ER stress [Iwawaki et al. 2003].
Materials in the Kit:
• Green/Red Ratiometric Cell Stress sensor BacMam
• Sodium Butyrate, 500 mM in H2O
• Thapsigargin (positive control)
Product Info & Protocols
|Protocol for Use|
We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. Purchases over $600 receive free shipping.
10mL, 30mL, 60mL
Lenti & AAV vectors available by special order
Lead times vary, please inquire!