Green Cell Stress Assay Kit #U0900G
$795.00 – $2,895.00
Bright fluorescent assay to detect response and reversal of chemical or genetic ER stress response and UPR. Can be combined with a red signaling sensor such as R-GECO to monitor disrupted signals in stressed cells. CMV Promoter.
The cell stress sensor is a genetically-encoded fluorescent biosensor that produces very bright green fluorescence when the cell endures endoplasmic reticulum (ER-mediated) stress or undergoes the unfolded protein response (UPR). Importantly, green fluorescent signals are nuclear localized for simple cell segmentation and image analysis. This sensor is based on the XBP1 mRNA which is spliced by the IRE1 protein during ER stress [Iwawaki et al. 2003]. Additional details may be found on the Cell Stress Assay page.
Materials in the Kit:
• Green Cell Stress Sensor BacMam
• Sodium Butyrate, 500 mM in H2O
• Thapsigargin 100 μM (control)
Additional information
| Protocol for Use | https://montanamolecular.com/cell-stress-assay-kit/cell-stress-sensor-protocol/ |
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| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| MSDS | |
| More Info | |
| Scientific Publications | |
| Kit Volume | 10mL, 30mL, 60mL |
| Terms of Sale |
- M. Ho, et al. Molecular mechanisms involved in alcohol craving, IRF3, and endoplasmic reticulum stress: a multi-omics study. Nature Translational Psychiatry. March 2024.
- L. Scheherazade Milane. Combination Organelle Mitochondrial Endoplasmic Reticulum Therapy (COMET) for Multidrug Resistant Breast Cancer. Journal of Controlled Release. October 2023.
- S. Sanchez-Martinez, et al. Labile assembly of a tardigrade protein induces biostasis. bioRxiv. July 2023.
- N. Mohammed, et al. Mechanisms of cell death induced by hexabromocyclododecane (HBCD) involves apoptosis, autophagy, and ER stress. Journal of Biochemical and Molecular Toxicology. June 2023.
- K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Frontiers Cellular Neuroscience. December 2019.
- K. Harlen, et al. Live-cell biosensors reveal novel insight into neurotoxic and cardiotoxic compound mediated cellular stress response. Journal of Pharmacological and Toxicological Methods. October 2019.
BioTek Application Note
Explore Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling
with Lionheart™ FX Automated Microscope
