Create full amylin pharmacology profiles using cAMP assays and titratable receptor components (CTR and RAMPs)
BacMam-packaged calcitonin receptor (CTR) and RAMP1–3 enable expression of functional amylin receptor subtypes (AMY1–3) in mammalian cells. BacMam provides tunable expression, allowing the CTR:RAMP ratio to be rapidly optimized for maximal conversion to amylin receptor pharmacology. When paired with the cADDis cAMP assay, these reagents provide a robust platform supporting amylin receptor target validation, compound profiling, and drug discovery. View example data and download a report below.
Summary
- cAMP generation by calcitonin family receptors was measured in COS-7 cells using the cADDis fluorescent cAMP biosensor in the BacMam expression system.
- The components of the receptor (GPCR and RAMP) were also expressed using BacMam, as follows:
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- Calcitonin receptor (CTR) – CTR alone
- AMY1 amylin receptor – CTR + RAMP1
- AMY2 amylin receptor – CTR + RAMP2
- AMY3 amylin receptor – CTR + RAMP3
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- The BacMam system enabled titration of RAMPs in assay development to ensure maximal conversion to the amylin pharmacology.
- cAMP stimulation in response to human calcitonin (hCT) and rat amylin (rAmy) was observed, with the expected potency.
Optimizing the CTR:RAMP Ratio
Peak cAMP results summary
Get time course data for AMY1R, AMY2R, AMY3R, methods, and optimization steps in the full report.
BacMam vectors to express RAMPs, calcitonin receptor (CTR), and the cADDis cAMP biosensor are available from Montana Molecular.
Profiling of your compounds for amylin receptor pharmacology is also offered through our CRO services. Contact us to discuss your project.