Live Cell Painting of iPSC-derived neurons

BacMam delivers live cell painting tools to BrainXell’s iPSC cortical glutamatergic neurons

Optimal expression does not involve HDAC inhibitors

A synapsin promoter driven reporter reveals neuronal differentiation in BrainXell iPSC glutamatergic neurons

iPSC derived cortical glutamatergic neurons from BrainXell (BX-0300-30)

Montana Molecular Paint Tools expressed in live iPSC derived neurons to visualize sub-cellular structures and cell morphology

Tau

Green TauPaint expresses the bright mNeon Green fluorescent protein fused to Tau.

Red TauPaint expresses the bright Montana Paintbrush Red fluorescent protein fused to Tau.

Tau

A co-transduction of Green H2BPaint (#P1020G) labeling the nucleus and Red TauPaint (#P1050R) labeling Tau in iPSC-derived neurons

Tau

Green TauPaint expresses the bright mNeon Green fluorescent protein fused to Tau.

Mitochondria

Green MiotPain (#P1010G) or Red MitoPaint (#P1010R) fluorescent proteins targeted to the mitochondria using the Cox8 motif.

Mitochondria

Green MitoPaint in live iPSC derived glutamatergic neurons

Lysosome

Red Lamp2Paint (#P1030R) shown here, or Green Lamp2Paint (#P1030G) targets fluorescent proteins to the lysosome using Lamp2.

Lysosome

Red Lamp2Paint (#P1030R) shown here, or Green Lamp2Paint (#P1030G) targets fluorescent proteins to the lysosome using Lamp2.

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1)

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1)

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1)

More iPSC Applications

Learn more about using BacMam to express genes of interest, label organelles, or measure stress in live iPSCs

Live Cell Painting of iPSC-derived neurons

BacMam delivers live cell painting tools to BrainXell’s iPSC cortical glutamatergic neurons

Optimal expression does not involve HDAC inhibitors

A synapsin promoter driven reporter reveals neuronal differentiation in BrainXell iPSC glutamatergic neurons

Montana Molecular Paint Tools expressed in live iPSC derived neurons to visualize sub-cellular structures and cell morphology

Tau

Tau

Tau

Mitochondria

Mitochondria

Lysosome

Lysosome

Omegasome

Omegasome

Omegasome

More iPSC Applications

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