Live Cell Painting in iPSC-derived neurons

Why use Montana Molecular’s Live Cell Paints?

  • Visualize subcellular function with the brightest fluorescent proteins in living cells
  • Monitor spatial and temporal changes in cell morphology and activity
  • Identify healthy and diseased phenotypes in living cells
  • Assess changes in phenotype in response to drug treatment or genetic modifications
  • Target expression to specific cell types in mixed cultures
  • Preserve cell viability – No fixing, no staining, no lysing
  • Packaged in our optimized BacMam viral delivery system
Why use a BacMam expression system?
  • BacMam provides robust expression in neuronal models including primary and iPSC-derived neurons
  • Low-toxicity and expression for 2-3 weeks in non-dividing cells
  • Highly consistent cell-cell and well-well expression
  • Easily titratable expression for rapid assay optimization
  • HDAC inhibitors were not necessary to achieve expression in BrainXell’s iPSC-derived glutametergic neurons

 

Montana Molecular Paint Tools expressed in live iPSC derived neurons to visualize sub-cellular structures and cell morphology

iPSC-derived cortical glutamatergic neurons from BrainXell (BX-0300-30)

Tau

Green TauPaint expresses the bright mNeon Green fluorescent protein fused to the Tau protein.

Red TauPaint expresses the bright Montana Paintbrush Red fluorescent protein fused to the Tau protein.

Tau

A co-transduction of Green H2BPaint (#P1020G) labeling the nucleus and Red TauPaint (#P1050R) labeling the Tau protein in iPSC-derived neurons

Tau

Green TauPaint expresses the bright mNeon Green fluorescent protein fused to the Tau protein.

Mitochondria

Green MitoPaint (#P1010G) or Red MitoPaint (#P1010R) fluorescent proteins targeted to the mitochondria

Mitochondria

Green MitoPaint in live iPSC derived glutamatergic neurons

Lysosome

Red Lamp2Paint (#P1030R) shown here, or Green Lamp2Paint (#P1030G) targets bright fluorescent proteins to the lysosome

Lysosome

Red Lamp2Paint (#P1030R) shown here, or Green Lamp2Paint (#P1030G) targets bright fluorescent proteins to the lysosome

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome 

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome 

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome

More iPSC Applications

Learn more about using BacMam to express genes of interest, label organelles, or measure stress in live iPSCs

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