Live Cell Painting of iPSC-derived neurons
Label organelles in live iPSC-derived neurons with fluorescent proteins
Why use Montana Molecular's Paints?
- Label organelles and subcellular domains with fluorescent proteins in living cells
- Monitor spatial and temporal changes in cell morphology and activity
- Identify healthy and diseased phenotypes in living cells
- Assess changes in phenotype or function response to drug treatment or genetic modifications
- Target expression to specific populations in mixed cultures with cell-specific promoters
- No need to fix, stain, or lyse
Why use a BacMam expression system?
- BacMam provides robust expression in neuronal models including primary and iPSC-derived neurons
- Low-toxicity and expression for 2-3 weeks in non-dividing cells
- Highly consistent cell-cell and well-well expression
- Easily titratable expression for rapid assay optimization
- HDAC inhibitors were not necessary to achieve expression in BrainXell’s iPSC-derived glutametergic neurons
iPSC-derived cortical glutamatergic neurons from BrainXell (BX-0300-30)
Montana Molecular Paint Tools expressed in live iPSC derived neurons to visualize sub-cellular structures and cell morphology
More iPSC Applications
Learn more about using BacMam to express genes of interest, label organelles, or measure stress in live iPSCs