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Live Cell Painting of iPSC-derived neurons

 

 

Label organelles in live iPSC-derived neurons with fluorescent proteins

Why use Montana Molecular's Paints?
  • Label organelles and subcellular domains with fluorescent proteins in living cells
  • Monitor spatial and temporal changes in cell morphology and activity
  • Identify healthy and diseased phenotypes in living cells
  • Assess changes in phenotype or function response to drug treatment or genetic modifications
  • Target expression to specific populations in mixed cultures with cell-specific promoters
  • No need to fix, stain, or lyse
Why use a BacMam expression system?
  • BacMam provides robust expression in neuronal models including primary and iPSC-derived neurons
  • Low-toxicity and expression for 2-3 weeks in non-dividing cells
  • Highly consistent cell-cell and well-well expression
  • Easily titratable expression for rapid assay optimization
  • HDAC inhibitors were not necessary to achieve expression in BrainXell’s iPSC-derived glutametergic neurons

iPSC-derived cortical glutamatergic neurons from BrainXell (BX-0300-30)

 

Montana Molecular Paint Tools expressed in live iPSC derived neurons to visualize sub-cellular structures and cell morphology

Tau

Green TauPaint expresses the bright mNeon Green fluorescent protein fused to Tau.

Red TauPaint expresses the bright Montana Paintbrush Red fluorescent protein fused to Tau.

Tau

A co-transduction of Green H2BPaint (#P1020G) labeling the nucleus and Red TauPaint (#P1050R) labeling Tau in iPSC-derived neurons

Tau

Green TauPaint expresses the bright mNeon Green fluorescent protein fused to Tau.

Mitochondria

Green MiotPain (#P1010G) or Red MitoPaint (#P1010R) fluorescent proteins targeted to the mitochondria using the Cox8 motif.

Mitochondria

Green MitoPaint in live iPSC derived glutamatergic neurons

Lysosome

Red Lamp2Paint (#P1030R) shown here, or Green Lamp2Paint (#P1030G) targets fluorescent proteins to the lysosome using Lamp2.

Lysosome

Red Lamp2Paint (#P1030R) shown here, or Green Lamp2Paint (#P1030G) targets fluorescent proteins to the lysosome using Lamp2.

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1)

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1)

Omegasome

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1)

More iPSC Applications

Learn more about using BacMam to express genes of interest, label organelles, or measure stress in live iPSCs

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