• A. Hamilton, et al. Nicotinic signaling stimulates glucagon secretion in mouse and human pancreatic α-cells. Diabetes. October 2024.
• X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. Molecular Biology of the Cell. May 2024. (bioRxiv)
• S. Datta, et al. APOL1-mediated monovalent cation transport contributes to APOL1-mediated podocytopathy in kidney disease. The Journal of Clinical Investigation. January 2024. (Supplemental Data)
• Z. Miller, et al. Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14. Cell Reports. November 2023. (bioRxiv)
• N. Zaïmia, et al. GLP-1 and GIP receptors signal through distinct β-arrestin 2-dependent pathways to regulate pancreatic β cell function. Cell Reports. October 2023.
• G. Sanchez, et al. Coincident Regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid Receptors Opposes Opioid-Mediated Antinociception. Molecular Pharmacology. December 2022.
• Z. Miller, et al. Lidocaine Induces Apoptosis in Head and Neck Squamous Cell Carcinoma Cells Through Activation of Bitter Taste Receptor T2R14. bioRxiv. November 2022.
• M. Doepner, et al. Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling. Science Advances. September 2022.
• A. Kim, et al. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes. eLife. November 2021. (bioRxiv)
• S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
• E. Maguire, et al. The Alzheimer's disease protective P522R variant of PLCG2, consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function. bioRxiv. April 2020.
• L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.
• S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
• C Deisl et al. Hypertrophy of human embryonic stem cell–derived cardiomyocytes supported by positive feedback between Ca2+ and diacylglycerol signals. Pflügers Archiv – European Journal of Physiology Jun. 2019
• Herranz, Gonzalo et al. Protein Kinase C δ Regulates the Depletion of Actin at the Immunological Synapse Required for Polarized Exosome Secretion by T Cells. Frontiers in Immunology Apr. 2019
• Ohno, et al. Dynamics of Presynaptic Diacylglycerol in a Sensory Neuron Encode Differences between Past and Current Stimulus Intensity. Cell Reports, Vol. 20, Issue 10. Sept. 2017
• Q. Wang, et al. Regulator of G protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion. FASEB J. Jul. 7,2017.
• Shigeto, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clinical Invest. 2015. doi:10.1172/JCI81975.
• Xingjuan, et al. PKC-dependent Phosphorylation of the H1 Histamine Receptor Modulates TRPC6 Activity. Cells. 2014.
• P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 2015. Request Full Text PDF
• Arttamangkul, et al.Does PKC activation increase the homologous desensitization of μ opioid receptors? British Journal of Pharmacology April 2014
• Tewson, et al. Simultaneous Detection of Ca2+and Diacylglycerol Signaling in Living Cells. PLoS One. 2012.

• L. Brueggemann, et al. Structural Determinants of Kv7.5 Potassium Channels that Confer Changes in Phosphatidylinositol 4,5-Biphosphate (PIP₂) Affinity and Signaling Sensitivity in Smooth Muscle Cells. Molecular Pharmacology. March 2020.
• L.Liu, et al. Gαq sensitizes TRPM8 to inhibition by PI(4,5)P2 depletion upon receptor activation. J.Neurosci. May 2019.
• Alexey N. Pronin, Qiang Wang and Vladlen Z. Slepak Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor Molecular Pharmacology Nov. 2018
• Y. Ding, et al. Ratiometric biosensors based on dimerization dependent fluorescent protein exchange. Nature Methods, 2015.
• P. Tewson, et al. A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. J. Biomolecular Screening 18, 2013.

• X. Chen, et al. In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection. EMBO Molecular Medicine. July 2024.
• X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. Molecular Biology of the Cell. May 2024. (bioRxiv)
• C. Amos, et al. Membrane lipids couple synaptotagmin to SNARE-mediated granule fusion in insulin-secreting cells. Molecular Biology of the Cell. December 2023.
• J. Wu, et al. Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal Cell Excitability and Working Memory. bioRxiv. March 2023.
• M. Thomas, et al. Optically activated, customizable, excitable cells. PLOS One. December 2020.
• L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.
• S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
• K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Front. Cell. Neurosci.,December 2019
• M.C. Cañizal, et al. A Dual Ca²⁺/ DAG Sensor Reports on Ligand Efficiency.
• C. Xingjuan, et al. PKC-dependent Phosphorylation of the H1 Histamine Receptor Modulates TRPC6 Activity. Cells. 2014.
• P. Tewson, et al. A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells. Journal of Biomolecular Screening 18, 2013.
• J. Wu, et al. Improved Orange and Red Ca²⁺ Indicators and Photophysical Considerations for Optogenetic Applications. ACS Chem Neurosci. Jun. 19, 2013
• H.J. Carlson, et al. Mutational Analysis of a Red Fluorescent Protein-Based Calcium Ion Indicator. Sensors, 2013.
• P. Tewson, et al. Simultaneous Detection of Ca²⁺ and Diacylglycerol Signaling in Living Cells. PLoS One. 2012.
•  

• JJ Bakhos, et al. Inhibition of Atrial Natriuretic Peptide Clearance Reduces Myocardial Fibrosis and Improves Cardiac Function in Diabetic Rats. bioRxiv. August 2024.
• RJ Lee, et al. Effects of Akt Activator SC79 on Human M0 Macrophage Phagocytosis and Cytokine Production. Cells. May 2024.
• R. Carey, et al. HSP90 Function is Required for T2R Bitter Taste Receptor Nitric Oxide Production and Innate Immune Responses in Human Airway Epithelial Cells and Macrophages. Cells. April 2022. (bioRxiv)
• I. Gapallawa, et al. Bitter taste receptors stimulate phagocytosis in human macrophages through calcium, nitric oxide, and cyclic-GMP signaling Cell. Mol. Life Sci. Feb, 2020 (bioRxiv)

• B. Dhayalan, et al. Synthetic Studies of the Mutant Proinsulin Syndrome Demonstrate Correlation Between Folding Efficiency and Age of Diabetes Onset. International Journal of Peptide Research and Therapeutics. November 2024.
• M. Ho, et al. Molecular mechanisms involved in alcohol craving, IRF3, and endoplasmic reticulum stress: a multi-omics study. Nature Translational Psychiatry. March 2024.
• L. Scheherazade Milane. Combination Organelle Mitochondrial Endoplasmic Reticulum Therapy (COMET) for Multidrug Resistant Breast Cancer. Journal of Controlled Release. October 2023.
• S. Sanchez-Martinez, et al. Labile assembly of a tardigrade protein induces biostasis. bioRxiv. July 2023.
• N. Mohammed, et al. Mechanisms of cell death induced by hexabromocyclododecane (HBCD) involves apoptosis, autophagy, and ER stress. Journal of Biochemical and Molecular Toxicology. June 2023.
• K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Frontiers Cellular Neuroscience. December 2019.
• K. Harlen, et al. Live-cell biosensors reveal novel insight into neurotoxic and cardiotoxic compound mediated cellular stress response. Journal of Pharmacological and Toxicological Methods. October 2019.

• A. Ippolito, et al. Increased 5-HT2A receptor signalling efficacy differentiates serotonergic psychedelics from non-psychedelics. bioRxiv. June 2024.
• H. Schiff, et al. β-arrestin-biased proteinase-activated receptor-2 antagonist C781 limits allergen-induced airway hyperresponsiveness and inflammation. British Journal of Pharmacology. June 2022.
• S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
• S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.
• S. Hoare, et al. Analyzing kinetic signaling data for G-protein-coupled receptors. Nature Scientific Reports. July 2020.
• S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports. Feb 2020.

• V. Fuochi, et al. Antiviral efficacy of heparan sulfate and enoxaparin sodium against SARS-CoV-2. Arch Pharm. November 2024.
• I. Gkekas, et al. AI Promoted Virtual Screening, Structure-Based Hit Optimization, and Synthesis of Novel COVID-19 S-RBD Domain Inhibitors. Journal of Chemical Information and Modeling. November 2024.
• D. Thomas, et al. CCL2-mediated endothelial injury drives cardiac dysfunction in long COVID. Nature Cardiovascular Research. October 2024.
• L. Monteonofrio. Molecular mechanisms of thalidomide effectiveness on COVID-19 patients explained: ACE2 is a new ΔNp63α target gene. Journal of Molecular Medicine. September 2024.
• A. Atemin. Kinetic landscape of single virus-like particles highlights the efficacy of SARS-Cov-2 internalization. bioRxiv. June 2024.
• J. Elste, et al. Co-Expression of Niemann-Pick Type C1-Like1 (NPC1L1) with ACE2 Receptor Synergistically Enhances SARS-CoV-2 Entry and Fusion. biomedicines. April 2024.
• Z. Wang, et al. Inhalation of ACE2-expressing lung exosomes provides prophylactic protection against SARS-CoV-2. nature communications. March 2024.
• F. Chen, et al. Hypercapnia increases ACE2 expression and pseudo-SARS-CoV-2 entry in bronchial epithelial cells by augmenting cellular cholesterol. Frontiers in Immunology. October 2023.
• Y. Kyosei, et al. Removal of soluble ACE2 in VeroE6 cells by 17β-estradiol reduces SARS-CoV-2 infectivity. Biological and Pharmaceutical Bulletin. October 2023.
• V. Fuochi, et al. Antiviral Efficacy of Heparan Sulfate and Enoxaparin Sodium against SARS-CoV-2: An In-Vitro/in-Silico Model. Preprints. October 2023.
• S. Shahbaz, et al. Analysis of SARS-CoV-2 isolates, namely the Wuhan strain, Delta variant, and Omicron variant, identifies differential immune profiles. Microbiology Spectrum. September 2023.
• J. Pérez-Vargas, et al. A novel class of broad-spectrum active-site-directed 3C-like protease inhibitors with nanomolar antiviral activity against highly immune-evasive SARS-CoV-2 Omicron subvariants. Emerging Microbes & Infections. August 2023.
• M. Farrag, et al. Structural requirements of Holothuria floridana fucosylated chondroitin sulfate oligosaccharides in anti-SARS-CoV-2 and anticoagulant activities. PLoS One. May 2023.
  A. Maurya, et al. Structure, anti-SARS-CoV-2, and anticoagulant effects of two sulfated galactans from the red alga Botryocladia occidentalis. International Journal of Biological Macromolecules. March 2023.
• X. Mei, et al. An inhaled bioadhesive hydrogel to shield non-human primates from SARS-CoV-2 infection. Nature Materials. February 2023.
• M. Spampinato, et al. Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells. Pharmaceutics. December 2022.
• S. Huang, et al. Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell Entry. International Journal of Molecular Sciences. November 2022.
• S. Shahbaz, et al. Differential effects of age, sex and dexamethasone therapy on ACE2/TMPRSS2 expression and susceptibility to SARS-CoV-2 infection. Frontiers in Immunology. November 2022.
• R. Dwivedi, et al. Anti-SARS-CoV-2 and anticoagulant properties of Pentacta Pygmaea fucosylated chondroitin sulfate depend on high molecular weight structures. Glycobiology. September 2022.
• A. Puhl, et al. Vandetanib Blocks the Cytokine Storm in SARS-CoV-2 Infected Mice. ACS Omega. August 2022.
• S.T. Chuang, P. Buchwald. Broad-Spectrum Small-Molecule Inhibitors of the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction from a Chemical Space of Privileged Protein Binders. Pharmaceuticals. August 2022.
• B.J. Travis, et al. Significance of chlorine-dioxide based oral rinses in preventing SARS-CoV-2 cell entry. Oral Diseases. July 2022.
• R. Dwivedi, et al. Inhibition of SARS-CoV-2 wild-type (Wuhan-Hu-1) and Delta (B.1.617.2) strains by marine sulfated glycans. Glycobiology. July 2022.
• Z. Wang, et al. Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine. Nature Biomedical Engineering. July 2022.
• BR Jo, et al. A Novel Antiviral Protein Derived from Oenanthe javanica: Type I Interferon-Dependent Antiviral Signaling and Its Pharmacological Potential. biomolecules. June 2022.
• S. Chuang, et al. Methylene Blue Is a Non-Specific Protein-Protein Interaction Inhibitor with Potential for Repurposing as Antiviral for COVID-19. Pharmaceuticals. May 2022.
• L. Souza-Moreira, et al. Poly(I:C) Enhances Mesenchymal Stem Cell Control of Myeloid Cells from COVID-19 Patients. iScience. April 2022.
• A. Puhl, et al. Vandetanib Reduces Inflammatory Cytokines and Ameliorates COVID-19 in Infected Mice. bioRxiv. December 2021.
• R. Dwivedi, et al. Structural and kinetic analyses of holothurian sulfated glycans suggest potential treatment for SARS-CoV-2 infection. Journal of Biological Chemistry. September 2021.
• A. Citarella, et al. SARS-CoV-2 M^pro: A Potential Target for Peptidomimetics and Small-Molecule Inhibitors. biomolecules. September 2021.
• Q. Zhang, et al. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience. July 2021.
• D. Bojadzic, et al. Small-Molecule Inhibitors of the Coronavirus Spike: ACE2 Protein-Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2. ACS Infectious Diseases. May 2021.
• A. Riad, et al. Differential gene expression by RNA-Seq in Sigma-2 Receptor/TMEM97 knockout cells reveals its role in complement activation and SARS-CoV-2 viral uptake. bioRxiv. March 2021.
• D. Bojadzic, et al. Methylene Blue Inhibits the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction-a Mechanism that can Contribute to its Antiviral Activity Against COVID-19. Frontiers in Pharmacology. January 2021.
• Pramanik, et al. The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles. Nanoscale Advances. January 2021.

ArcLight Voltage Sensor (#D0700G)

• M. Thomas, et al. Optically activated, customizable, excitable cells. PLOS One. December 2020.

GPCRs in BacMam

• S. Shi, et al. A high-affinity, cis-on photoswitchable beta blocker to optically control β2-adrenergic receptors in vitro and in vivo. Biochemical Pharmacology. June 2024.
• S. Fulton, et al. Characterizing a new tool to manipulate area postrema GLP1R+ neurons across species. Physiology & Behavior. January 2024.
• M. Nuriya, et al. Alkyne-Tagged Dopamines as Versatile Analogue Probes for Dopaminergic System Analysis. Analytical Chemistry. July 2021.
• T. Vien, et al. Molecular dysregulation of ciliary polycystin-2 channels caused by variants in the TOP domain. PNAS. April 2020.

Embryonic Stem Cell-Derived Cardiomyocytes (Human)

• C Deisl et al. Hypertrophy of human embryonic stem cell–derived cardiomyocytes supported by positive feedback between Ca2+ and diacylglycerol signals. Pflügers Archiv – European Journal of Physiology Jun. 2019

iPSC-derived Cardiomyocytes

• K. Harlen, et al. Live-cell biosensors reveal novel insight into neurotoxic and cardiotoxic compound mediated cellular stress response. Journal of Pharmacological and Toxicological Methods. October 2019.

Rat Ventricular Cardiac Fibroblasts

• JJ Bakhos, et al. Inhibition of Atrial Natriuretic Peptide Clearance Reduces Myocardial Fibrosis and Improves Cardiac Function in Diabetic Rats. bioRxiv. August 2024.

Pancreatic Islets (Human)

• A. Hamilton, et al. Nicotinic signaling stimulates glucagon secretion in mouse and human pancreatic α-cells. Diabetes. October 2024.
• N. Zaïmia, et al. GLP-1 and GIP receptors signal through distinct β-arrestin 2-dependent pathways to regulate pancreatic β cell function. Cell Reports. October 2023.
• J. Cho, et al. Islet primary cilia motility controls insulin secretion. Science Advances. September 2022. (bioRxiv)
• J. Almaça, et al. Human beta cells produce and release serotonin to inhibit glucagon secretion from alpha cells.Cell Reports, Volume 17, Issue 12, 2016.

Pancreatic Islets (Mouse)

• LE Eid, et al. In vivo functional profiling and structural characterisation of the human Glp1r A316T variant. bioRxiv. October 2024.
• A. Oqua, et al. Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells. bioRxiv. June 2024.
• C. Hinds, et al. Abolishing β-arrestin recruitment is necessary for the full metabolic benefits of G protein-biased glucagon-like peptide-1 receptor agonists. Diabetes, Obesity and Metabolism. October 2023.
• N. Zaïmia, et al. GLP-1 and GIP receptors signal through distinct β-arrestin 2-dependent pathways to regulate pancreatic β cell function. Cell Reports. October 2023.
• S. Bitsi, et al. Divergent acute versus prolonged pharmacological GLP-1R responses in adult β cell–specific β-arrestin 2 knockout mice. Science Advances. May 2023.
• S. Bitsi, et al. Divergent acute versus prolonged in vivo GLP-1R responses in β-arrestin 2-deleted primary beta cells. bioRxiv. April 2022.
• A. Kim, et al. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes. eLife. November 2021. (bioRxiv)
• M. Draper, et al. Imaging Meets Cytometry: Analyzing Heterogenous Functional Microscopic Data from Living Cell Populations. J. Imaging. January 2021.
• N. Daram, et al. Glucagon-like Peptide 1 Shows Glucose Dependence in Regulating Islet Hormone Secretion. Metabolism. March 2020.
• M. Marasco et al. Interleukin-6 Reduces b-Cell Oxidative Stress by Linking Autophagy With the Antioxidant Response. Diabetes. August 2018.
• A. Hamilton et al. Adrenaline Stimulates Glucagon Secretion by Tpc2-Dependent Ca2+ Mobilization From Acidic Stores in Pancreatic α-Cells. Diabetes. June 2018.
• M. Shigeto, et al. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clinical Invest. 2015. doi:10.1172/JCI81975.

α-Cells (Mouse)

• J. Knudsen, et al. Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production. Cell Metabolism. February 2019.

Airway Smooth Muscle (Human, HASM)

• I. Cattani-Cavalieri, et al. Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ. Journal of Visualized Experiments. March 2024.
• P. Balraj, et al. Kisspeptin/KISS1R Signaling Modulates Human Airway Smooth Muscle Cell Migration. American Journal of Respiratory Cell and Molecular Biology. March 2024.
• I. Cattani-Cavalieri, et al. Quantitative phosphoproteomic analysis reveals unique cAMP signaling pools emanating from AC2 and AC6 in human airway smooth muscle cells. Frontiers in Physiology. February 2023.
• C. Koziol-White et al. Budesonide enhances agonist-induced bronchodilation in human small airways by increasing cAMP production in airway smooth muscle. American Journal of Physiology. February 2020.
• Nunez, F.J., Johnstone, T.B. et al. Glucocorticoids rapidly activate cAMP production via Gαs to initiate non‐genomic signaling that contributes to one‐third of their canonical genomic effects FASEB Journal, Dec. 2019
• C. Ojiaku, et al. Transforming Growth Factor-β1 Decreases β2-Agonist–induced Relaxation in Human Airway Smooth Muscle ATSJournas, Dec 2018
• T.B. Johnstone, et al. PDE8 is Expressed in Human Airway Smooth Muscle and Selectively Regulates cAMP Signaling by β2AR-AC6. American Journal of Respiratory Cell and Molecular Biology. Dec. 2017.

Bronchial Epithelial (Human hBECs)

• F. Chen, et al. Hypercapnia increases ACE2 expression and pseudo-SARS-CoV-2 entry in bronchial epithelial cells by augmenting cellular cholesterol. Frontiers in Immunology. October 2023.

Lung Microvascular Endothelial (Rat)

• N. Philip. Fatty acid metabolism promotes TRPV4 activity in lung microvascular endothelial cells in pulmonary arterial hypertension. Lung Cellular and Molecular Physiology. January 2024.

Primary Human Uterine Smooth Muscle Cells (Human, HUSMC)

• X. Chen, et al. Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling. Scientific Reports Aug. 2018.

Primary Mouse Uterine Smooth Muscle Cells (Mouse, MUSMC)

• X. Chen, et al. Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling. Scientific Reports Aug. 2018.

Primary Human Macrophage

• RJ Lee, et al. Effects of Akt Activator SC79 on Human M0 Macrophage Phagocytosis and Cytokine Production. Cells. May 2024.

Odontoblasts

• N. Saito, et al. Gα_s-Coupled CGRP Receptor Signaling Axis from the Trigeminal Ganglion Neuron to Odontoblast Negatively Regulates Dentin Mineralization. biomolecules. November 2022.

Osteoarthritis synoviocytes

• M. Qadri et al. cAMP attenuates TGF-β’s profibrotic responses in osteoarthritic synoviocytes: involvement of hyaluronan and PRG4. Cell Physiology. September 2018.

Nasal Gland Serous Cells (Acinar)

• D. McMahon, et al. Neuropeptide regulation of secretion and inflammation in human airway gland serous cells European Respiratory Journal. Feb 2020 (bioRxiv)

Muscle Stem Cells (MuSCs)

• E. Porpiglia, et al. Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration. Cell Stem Cell. December 2022. (bioRxiv)

CD1 Mice

• X. Mei, et al. An inhaled bioadhesive hydrogel to shield non-human primates from SARS-CoV-2 infection. Nature Materials. February 2023.
• Z. Wang, et al. Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine. Nature Biomedical Engineering. July 2022.

Dental Pulp Slices

• N. Saito, et al. Gα_s-Coupled CGRP Receptor Signaling Axis from the Trigeminal Ganglion Neuron to Odontoblast Negatively Regulates Dentin Mineralization. biomolecules. November 2022.

A7r5 (Rat Smooth Muscle Embryonic Aorta)

• L. Brueggemann, et al. Structural Determinants of Kv7.5 Potassium Channels that Confer Changes in Phosphatidylinositol 4,5-Biphosphate (PIP₂) Affinity and Signaling Sensitivity in Smooth Muscle Cells. Molecular Pharmacology. March 2020.

H9C2 (Rat Cardiomyocytes)

• K.A. McCrink, et al. β-Arrestin2 Improves Post-Myocardial Infarction Heart Failure via Sarco(endo)plasmic Reticulum Ca2+-ATPase-Dependent Positive Inotropy in Cardiomyocytes. Hypertension. Nov. 2017. (See Supplemental Materials)

HPAE (Human Pulmonary Artery Endothelial)

• D. Santana Nunez, et al. Piezo1 induces endothelial responses to shear stress via soluble adenylyl Cyclase-IP₃R2 circuit. iScience. May 2023.

EndoC-βH5 (β-cell model)

• C. Hinds, et al. Abolishing β-arrestin recruitment is necessary for the full metabolic benefits of G protein-biased glucagon-like peptide-1 receptor agonists. Diabetes, Obesity and Metabolism. October 2023.

INS-1 (GRINCH)

• C. Amos, et al. Membrane lipids couple synaptotagmin to SNARE-mediated granule fusion in insulin-secreting cells. Molecular Biology of the Cell. December 2023.

INS-1 832 (3 insulinoma cells25)

• Y. Manchange, et al. Engineered mini-G proteins block the internalization of cognate GPCRs and disrupt downstream intracellular signaling. Science Signaling. July 2024.
• A. Oqua, et al. Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells. bioRxiv. June 2024.
• G. Austin, et al. An inter-organelle contact between endosomal GLP-1R, ER VAP-B, and the mitochondrial AKAP SPHKAP triggers PKA-dependent MIC19 phosphorylation and β-cell mitochondrial remodelling. bioRxiv. April 2024.
• C. Hinds, et al. Abolishing β-arrestin recruitment is necessary for the full metabolic benefits of G protein-biased glucagon-like peptide-1 receptor agonists. Diabetes, Obesity and Metabolism. October 2023.

MIN6 

• C. Wu, et al. Discovery of ciliary G protein-coupled receptors regulating pancreatic islet insulin and glucagon secretion. Genes & Development. August 2021. bioRxiv.
• Q. Wang, et al. Regulator of G protein signaling Gβ5-R7 is a crucial activator of muscarinic M3 receptor-stimulated insulin secretion. FASEB J. Jul. 7,2017.

α-TC9

• C. Wu, et al. Discovery of ciliary G protein-coupled receptors regulating pancreatic islet insulin and glucagon secretion. Genes & Development. August 2021. bioRxiv.

16HBE (Human Bronchial Epithelial)

• H. Schiff, et al. β-arrestin-biased proteinase-activated receptor-2 antagonist C781 limits allergen-induced airway hyperresponsiveness and inflammation. British Journal of Pharmacology. June 2022.
• D. McMahon, et al. The bitter end: T2R bitter receptor agonists elevate nuclear calcium and induce apoptosis in non-ciliated airway epithelial cells. Cell Calcium. January 2022. (bioRxiv)

A549 (Adenocarinomic Human Alveolar Basal Epithelial Cells)

• V. Fuochi, et al. Antiviral efficacy of heparan sulfate and enoxaparin sodium against SARS-CoV-2. Arch Pharm. November 2024.
• Z. Wang, et al. Inhalation of ACE2-expressing lung exosomes provides prophylactic protection against SARS-CoV-2. nature communications. March 2024.
• V. Fuochi, et al. Antiviral Efficacy of Heparan Sulfate and Enoxaparin Sodium against SARS-CoV-2: An In-Vitro/in-Silico Model. Preprints. October 2023.
• M. Spampinato, et al. Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells. Pharmaceutics. December 2022.
• BR Jo, et al. A Novel Antiviral Protein Derived from Oenanthe javanica: Type I Interferon-Dependent Antiviral Signaling and Its Pharmacological Potential. biomolecules. June 2022.

H441 (Airway Epithelial Cells)

• R. Carey, et al. HSP90 Modulates T2R Bitter Taste Receptor Nitric Oxide Production and Innate Immune Responses in Human Airway Epithelial Cells and Macrophages. Cells. April 2022. (bioRxiv)

HAEpi (Human Pulmonary Alveoli Epithelial)

• L. Souza-Moreira, et al. Poly(I:C) Enhances Mesenchymal Stem Cell Control of Myeloid Cells from COVID-19 Patients. iScience. April 2022.

HFL-1 (Human Fetal Lung)

• Nunez, F.J., Johnstone, T.B. et al. Glucocorticoids rapidly activate cAMP production via Gαs to initiate non‐genomic signaling that contributes to one‐third of their canonical genomic effects FASEB Journal, Dec. 2019
• Nunez, F.J., Schulte, N.A., Fogel, D.M., et al. Agonist-specific desensitization of PGE2-stimulated cAMP signaling due to upregulated phosphodiesterase expression in human lung fibroblasts Naunyn-Schmiedeberg’s Arch Pharmacol Dec. 2019.

NHBE (Normal Human Bronchial Epithelial)

• S. Shahbaz, et al. Differential effects of age, sex and dexamethasone therapy on ACE2/TMPRSS2 expression and susceptibility to SARS-CoV-2 infection. Frontiers in Immunology. November 2022.

ADP293

• L. Bridge, et al. Computational modelling of dynamic cAMP responses to GPCR agonists for exploration of GLP-1R ligand effects in pancreatic β-cells and neurons. Cellular Signaling, April 2024.

BHK (Baby Hamster Kidney fibroblasts)

• X. Chen, et al. In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection. EMBO Molecular Medicine. July 2024.

COS-7 (Monkey Kidney Fibroblast-like cells)

• N. Mohammed, et al. Mechanisms of cell death induced by hexabromocyclododecane (HBCD) involves apoptosis, autophagy, and ER stress. Journal of Biochemical and Molecular Toxicology. June 2023.
• T. Baldwin, et al. Insights into the Regulatory Properties of Human Adenylyl Cyclase Type 9 Molecular Pharmacology. April, 2019

HEK293 (Human Embryonic Kidney)

• B. Dhayalan, et al. Synthetic Studies of the Mutant Proinsulin Syndrome Demonstrate Correlation Between Folding Efficiency and Age of Diabetes Onset. International Journal of Peptide Research and Therapeutics. November 2024.
• S. Shi, et al. A high-affinity, cis-on photoswitchable beta blocker to optically control β2-adrenergic receptors in vitro and in vivo. Biochemical Pharmacology. June 2024.
• R. Schuck, et al. Cholesterol inhibits assembly and activation of the EphA2 receptor. bioRxiv. June 2024.
• I. Cattani-Cavalieri, et al. Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ. Journal of Visualized Experiments. March 2024.
• B. Polacco, et al. Profiling the proximal proteome of the activated μ-opioid receptor. nature chemical biology. March 2024.
• J. Xu, et al. An evolutionarily conserved olfactory receptor is required for sex differences in blood pressure. Science Advances. March 2024 (bioRxiv)
• S. Datta, et al. APOL1-mediated monovalent cation transport contributes to APOL1-mediated podocytopathy in kidney disease. The Journal of Clinical Investigation. January 2024. (Supplemental Data)
• S. Fulton, et al. Characterizing a new tool to manipulate area postrema GLP1R+ neurons across species. Physiology & Behavior. January 2024.
• E. Blythe & M. von Zastrow. β-Arrestin-independent endosomal cAMP signaling by a polypeptide hormone GPCR. Nature Chemical Biology. September 2023.
• YJ Peng, et al. Hypoxia sensing requires H₂S-dependent persulfidation of olfactory receptor 78. Science Advances. July 2023.
• S. Sanchez-Martinez, et al. Labile assembly of a tardigrade protein induces biostasis. bioRxiv. July 2023.
• M. Farrag, et al. Structural requirements of Holothuria floridana fucosylated chondroitin sulfate oligosaccharides in anti-SARS-CoV-2 and anticoagulant activities. PLoS One. May 2023.
• V. Bhatia, et al. Characterization of Adenylyl Cyclase Isoform 6 Residues Interacting with Forskolin. Biology. April 2023.
• A. Maurya, et al. Structure, anti-SARS-CoV-2, and anticoagulant effects of two sulfated galactans from the red alga Botryocladia occidentalis. International Journal of Biological Macromolecules. March 2023.
• H. Carr, et al. The Wnt pathway protein Dvl1 targets Somatostatin receptor 2 for lysosome-dependent degradation. Journal of Biological Chemistry. March 2023.
• J. Wu, et al. Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal Cell Excitability and Working Memory. bioRxiv. March 2023.
• G. Sanchez, et al. Coincident Regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid Receptors Opposes Opioid-Mediated Antinociception. Molecular Pharmacology. December 2022.
• J. Janetzko, et al. Membrane phosphoinositides regulate GPCR-β-arrestin complex assembly and dynamics. Cell. November 2022.
• S. Huang, et al. Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell Entry. International Journal of Molecular Sciences. November 2022.
• B. Barsi-Rhyne, et al. Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling. eLife. October 2022. (bioRxiv)
• S. A. Dai, et al. State-selective modulation of heterotrimeric Gαs signaling with macrocyclic peptides. Cell. September 2022.
• R. Dwivedi, et al. Anti-SARS-CoV-2 and anticoagulant properties of Pentacta Pygmaea fucosylated chondroitin sulfate depend on high molecular weight structures. Glycobiology. September 2022.
• M. Doepner, et al. Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling. Science Advances. September 2022.
• E. Blythe, M. von Zastrow. A discrete mode of endosomal GPCR signaling that does not require β-arrestins. bioRxiv. September 2022.
• S.T. Chuang, P. Buchwald. Broad-Spectrum Small-Molecule Inhibitors of the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction from a Chemical Space of Privileged Protein Binders. Pharmaceuticals. August 2022.
• B.J. Travis, et al. Significance of chlorine-dioxide based oral rinses in preventing SARS-CoV-2 cell entry. Oral Diseases. July 2022.
• R. Dwivedi, et al. Inhibition of SARS-CoV-2 wild-type (Wuhan-Hu-1) and Delta (B.1.617.2) strains by marine sulfated glycans. Glycobiology. July 2022.
• J. Xu, J. Pluznick. Key amino acids alter activity and trafficking of a well-conserved olfactory receptor. Cell Physiology. June 2022.
• S. Chuang, et al. Methylene Blue Is a Non-Specific Protein-Protein Interaction Inhibitor with Potential for Repurposing as Antiviral for COVID-19. Pharmaceuticals. May 2022.
• Y. Mizobuchi, et al. Ketamine Improves Desensitization of μ-Opioid Receptors Induced by Repeated Treatment with Fentanyl but Not with Morphine. biomolecules. March 2022.
• B. Polacco, et al. Profiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors. bioRxiv. March 2022.
• S. Hoare, et al. Quantifying the Kinetics of Signaling and Arrestin Recruitment by Nervous System G-Protein Coupled Receptors. Frontiers in Cellular Neuroscience. January 2022.
• Y. Karasawa, et al. In Vitro Analyses of Spinach-Derived Opioid Peptides, Rubiscolins: Receptor Selectivity and Intracellular Activities through G-protein- and β-arrestin-Mediated Pathways. Molecules. October 2021.
• A. White, et al. Spatial Bias in cAMP generation determines biological responses to PTH type 1 receptor activation. Science Signaling. October 2021.
• J. Janetzko, et al. Membrane phosphoinositides stabilize GPCR-arrestin complexes and offer temporal control of complex assembly and dynamics. bioRxiv. October 2021.
• A. Citarella, et al. SARS-CoV-2 M^pro: A Potential Target for Peptidomimetics and Small-Molecule Inhibitors. biomolecules. September 2021.
• Y. Kuroda, et al. Inhibition of endothelin A receptor by a novel, selective receptor antagonist enhances morphine-induced analgesia: Possible functional interaction of dimerized endothelin A and μ-opioid receptors. Biomedicine & Pharmacotherapy. September 2021.
• R. Dwivedi, et al. Structural and kinetic analyses of holothurian sulfated glycans suggest potential treatment for SARS-CoV-2 infection. Journal of Biological Chemistry. September 2021.
• Q. Zhang, et al. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience. July 2021.
• A. Gad, et al. Conserved residues in extracellular loop 2 regulate Stachel-mediated activation of ADGRG2. Scientific Reports. July 2021. (bioRxiv)
• D. Bojadzic, et al. Small-Molecule Inhibitors of the Coronavirus Spike: ACE2 Protein-Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2. ACS Infectious Diseases. May 2021.
• H. Carr, et al. The PDZ Domain Protein SYNJ2BP Regulates GRK-dependent Sst2A Phosphorylation and Downstream MAPK Signaling. Endocrinology. February 2021.
• D. Bojadzic, et al. Methylene Blue Inhibits the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction-a Mechanism that can Contribute to its Antiviral Activity Against COVID-19. Frontiers in Pharmacology. January 2021.
• Pramanik, et al. The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles. Royal Society of Chemistry. January 2021.
• M. Thomas, et al. Optically activated, customizable, excitable cells. PLOS One. December 2020.
• S. Hoare, et al. Analyzing kinetic signaling data for G-protein-coupled receptors. Nature Scientific Reports. July 2020.
• T. Vien, et al. Molecular dysregulation of ciliary polycystin-2 channels caused by variants in the TOP domain.PNAS. April 2020.
• S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports. Feb 2020.
• Nunez, F.J., Johnstone, T.B. et al. Glucocorticoids rapidly activate cAMP production via Gαs to initiate non‐genomic signaling that contributes to one‐third of their canonical genomic effects FASEB Journal, Dec. 2019
• K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Front. Cell.
• L.Liu, et al. Gαq sensitizes TRPM8 to inhibition by PI(4,5)P2 depletion upon receptor activation. J.Neurosci. May 2019.
• [plasmid]Alexey N. Pronin, Qiang Wang and Vladlen Z. Slepak Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor Molecular Pharmacology Nov. 2018
• Y.A.B. Sierra, et al. Potassium channel-based optogenetic silencing. Nature Communications Nov. 5 2018.
• P. Tewson, et al. Assay for Detecting Gαi-Mediated Decreases in cAMP in Living Cells. SLAS. July 10, 2018. Request Full Text PDF
• P. Tewson, et al. New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories. J Biomol Screen Dec. 2015. Request Full Text PDF

HK-2 (Human Kidney 2)

• Q. Zhang, et al. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience. July 2021.

LL-CPK1 (Renal Epithelial)

• Sherpa et al. Sensory primary cilium is a responsive cAMP microdomain in renal epithelia. Scientific Reports. April 2019.

MCDK (Madin-Darby Canine Kidney)

• T. Togo Autocrine purinergic signaling stimulated by cell membrane disruption is involved in both cell membrane repair and adaptive response in MDCK cells Biochem & Biophysical Research Comm. March, 2019
• T. Togo Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells Biology Open. December 2017.

IMCD-3 (Mouse Inner Medullary Collecting Duct)

• S. Ansari, et al. Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length. Journal of Cell Biology. June 2024.
• R. Fagan, et al. Selective targeting of mu opioid receptors to primary cilia. Cell Reports. April 2024.
• J. Hansen, et al. A cAMP signalome in primary cilia drives gene expression and kidney cyst formation. EMBO Reports. June 2022.
• S. Ansari, et al. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv. May 2022.
• J. Hansen, et al. Nanobody-directed targeting of ontogenetic tools to study signaling in the primary cilium. eLife. June 2020.

Vero E6

• Y. Kyosei, et al. Removal of soluble ACE2 in VeroE6 cells by 17β-estradiol reduces SARS-CoV-2 infectivity. Biological and Pharmaceutical Bulletin. October 2023.
• S. Shahbaz, et al. Analysis of SARS-CoV-2 isolates, namely the Wuhan strain, Delta variant, and Omicron variant, identifies differential immune profiles. Microbiology Spectrum. September 2023.

3D HSG Acini (Human Salivary Gland)

• J. Cortes-Troncoso, et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjörgen’s Syndrome. JCI Insight. May 2020.

3T3-L1 (Mouse Fibroblasts/adipocyte-like cells)

• G. Sancar, et al. FGF1 and insulin control lipolysis by convergent pathways. Cell Metabolism. January 2022.
• K. Hilgendorf, et al. Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis. Cell. November 2019.

A431

• L. Monteonofrio. Molecular mechanisms of thalidomide effectiveness on COVID-19 patients explained: ACE2 is a new ΔNp63α target gene. Journal of Molecular Medicine.

BT-549

Caco-2 (Immortalized Human Colorectal Adenocarcinoma)

CHO (Chinese Hamster Ovary)

• H. Hamzeh, et al. Deciphering rapid cell signaling and control of cell motility by reverse opto-chemical engineering. bioRxiv. February 2024.
• M. Nuriya, et al. Alkyne-Tagged Dopamines as Versatile Analogue Probes for Dopaminergic System Analysis. Analytical Chemistry. July 2021.
• T. Buranda, et al. A High-Throughput Flow Cytometry Screen Identifies Molecules that Inhibit Hantavirus Cell Entry. SLAS Discovery. April 2, 2018.

HeLa (Cervical Adenocarcinoma)

• A. Riad, et al. Differential gene expression by RNA-Seq in Sigma-2 Receptor/TMEM97 knockout cells reveals its role in complement activation and SARS-CoV-2 viral uptake. bioRxiv. March 2021.

Huh7 (Heapatocyte Carcinoma)

• ER McGlone, et al. Hepatocyte cholesterol content modulates glucagon receptor signaling. Molecular Metabolism. September 2022.

Human Odontoblasts (HOB)

• M. Kimura, et al. Intracellular cAMP signaling-induced Ca2+ influx mediated by calcium homeostasis modulator 1 (CALHM1) in human odontoblasts. European Journal of Physiology. November 2024.
• E. Kitayama, et al. Functional Expression of IP, 5-HT₄, D₁, A_2A, and VIP Receptors in Human Odontoblast Cell Line. Biomolecules. May 2023.

HUVEC (Human Umbilical Vein Endothelia)

• A. Puhl, et al. Vandetanib Blocks the Cytokine Storm in SARS-CoV-2 Infected Mice. ACS Omega. August 2022.
• A. Puhl, et al. Vandetanib Reduces Inflammatory Cytokines and Ameliorates COVID-19 in Infected Mice. bioRxiv. December 2021.

LNCaP (Prostate Adenocarcinoma)

• A. Pronin, et al. Ectopically expressed olfactory receptors OR51E1 and OR51E2 suppress proliferation and promote cell death in a prostate cancer cell line. Journal of Biological Chemistry. February 2021.

M-MØP (Immortalized Macrophage Precursor)

• E. Maguire, et al. The Alzheimer’s disease protective P522R variant of PLCG2, consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function. bioRxiv. April 2020.

MDA-MB-231

• L. Scheherazade Milane. Combination Organelle Mitochondrial Endoplasmic Reticulum Therapy (COMET) for Multidrug Resistant Breast Cancer. Journal of Controlled Release. October 2023.

NIH3T3 (Mouse Fibroblasts)

• MP Pedro et al. Activation of G-Protein Coupled Receptor-Gai Signaling Increases Keratinocyte Proliferation and Reduces Differentiation, Leading to Epidermal Hyperplasia. Journal of Investigative Dermatology. November 2019.

SCC 47 (Squamous Cell Carcinoma)

• Z. Miller, et al. Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14. Cell Reports. November 2023. (bioRxiv)

T84 (Colorectal Carcinoma)

• R. Forteza, et al. Glucocorticoids and Myosin5b loss-of-function induce heightened PKA signaling in addition to membrane traffic defects. Molecular Biology of the Cell. December 2019.

U2OS

• A. Atemin. Kinetic landscape of single virus-like particles highlights the efficacy of SARS-Cov-2 internalization. bioRxiv. June 2024.