Big Sky BacMam Improves Transduction Efficiency

Big Sky BacMam Improves Transduction Efficiency

Big Sky BacMam is an improvement on Montana Molecular’s original “OG” BacMam vector. In our first experiments with HEK293T and CHO-K1 cells, we found Big Sky BacMam yielded significant boosts in expression. Montana Molecular’s R&D team and users of our fluorescent biosensors and other BacMam-packaged tools are continuing see improvements in new cell types, including iPSC-derived neurons and airway smooth muscle cells (ASMs).

Reach out to learn more about Big Sky BacMam or to try it in your cells!

Big Sky BacMam enabled expression in Airway smooth muscle cells (ASMs)

Courtesy of Jacob Tesch at North Dakota State University – a comparison of Green cADDis cAMP sensor expression in ASMs using the Big Sky vector (U0250G) and OG vector (U0200G).

iPSC-derived neurons are robustly transduced with Big Sky

Montana Paintbrush RFP expressed in iPSC-derived Glutamatergic Cortical neurons from BrainXell. Big Sky BacMam provided robust expression in these neurons even in the absence of HDAC inhibitors, which are commonly used to boost and maintain BacMam expression.

Interested in trying Big Sky BacMam in your cells? Looking to express one of our fluorescent biosensors, live cell painting tools, GPCRs, or a custom gene? Let us know below!