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Live Cell Painting Tools (Paints)

Montana Molecular’s live cell painting tools – Paints – enable visualization of organelles, subcellular structures, and cell morphology in the context of drug discovery and disease research. Use the bright, fluorescent tools to investigate drug effects, monitor metabolic activities, and identify disease-associated phenotypes.

  • Packaged in a BacMam viral vector for consistent, titratable expression in a wide range of cell types – including many primary and iPSC cultures
  • Easily co-transduce red and green fluorescent Paints, biosensors, and other BacMam-packaged constructs
  • Capture multiple timepoints in a single well
  • Label intracellular structures for phenotypic screens
  • No fixation, staining, or cell lysis required

 

Red NSLPaint co-transduced with Green MitoPaint

A co-transduction of Green H2BPaint (#P1020G) and Red TauPaint (#P1050R) in iPSC-derived neurons

Mitochondrial Labels (MitoPaint)

mNeon Green or Montana Paintbrush Red fluorescent proteins targeted to the mitochondria using the Cox8 motif.

 

 

Red MitoPaint (#P1010R) Expressed in HEK293s

Green MitoPaint (#P1010G) Expressed in HEK293s

Nuclear Labels

Red NLSPaint (#P1020R) expresses the Montana Paintbrush Red fluorescent protein targeted to the nucleus using an NLS targeting motif.

Green H2BPaint (#P1020G) expresses the mNeon Green fluorescent protein, targeted to the nucleus using Histone 2B (H2B).

mNeon Green targeted to the Endosome coexpressed with red NLSPaint

Red NLSPaint (#P1020R) Expressed in HEK293s along with Green Lamp2Paint (#P1030G)

Green H2BPaint (#P1020G) Expressed in HEK293s along with Red Lamp2Paint (#P1030R)

Lysosomal Labels (Lamp2Paint)

mNeon Green or Montana Paintbrush Red fluorescent proteins targeted to the lysosome using Lamp2. Red Lamp2Paint (#P1030R) is shown below, Green Lamp2Paint (#P1030G) is also available.

 

Red Lamp2Paint (#P1030R) and Green MitoPaint (#P1010G) Expressed in HEK293s

Golgi Labels (GiantinPaint)

Montana Paintbrush Red and mNeon Green fluorescent proteins targeted to the Golgi using Giantin.

Montana Paintbrush RFP expressed in HEK293 cells

Red GiantinPaint (#P1040R) expressed in HEK293 cells.

Green GiantinPaint (#P1040G) expressed in HEK293 cells along with Red NLSPaint (#P1020R).

Cytoskeletal Labels

Label cytoskeletal structures with mNeon Green and Montana Paintbrush Red fluorescent proteins fused to Tau, human Beta Actin, and Alpha Tubulin.

 

Green TauPaint (#P1050G) expressed in iPSC-derived neurons. Red TauPaint (#P1050R) is also available.

Red BetaActinPaint (#P1060R) expressed in iPSC-derived neurons with Green H2BPaint. Green BetaActinPaint (#P1060G) is also available.

Green AlphaTubulinPaint (#P1070G) expressed in iPSC-derived neurons. Red AlphaTubulinPaint (#P1070R) is also available.

Endosomal Label (EndoPaint)

EndoPaint is the bright mNeon Green fluorescent protein targeted to the endosome using FYVE.

 

Green EndoPaint (#P1080G) and Red NLSPaint (#P1020R) co-expressed in HEK293s

Omegasomal Label (OmegaPaint)

OmegaPaint is the bright mNeon Green fluorescent protein targeted to the omegasome using the double FYVE-containing protein 1 (ZNFN2A1).

 

Green OmegaPaint (#P1090G) and Red NLSPaint (#P1020R) co-expressed in HEK293s

Untargeted Fluorescent Proteins

Montana Paintbrush Red and mNeon Green are extremely bright, rapidly folding fluorescent proteins. They are ideal for evaluating transduction efficiency and optimizing expression in your cell type of choice.
Montana Paintbrush RFP expressed in HEK293 cells

Montana Paintbrush fluorescent protein (#P1000R) expressed in HEK293 cells.

mNeon Green fluorescent protein (#P1000G) expressed in HEK293 cells.

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