GENIe:
Live Cell cGMP Assays
Detect cGMP signaling with GENIe
- GENIe is a genetically-encoded fluorescent cGMP assay
- Measure cGMP signaling in real time with a single sensor
- Study cGMP production or PDE inhibition
- Screen for PDE inhibitors
- Examine PDE Biology
- Monitor fluorescence changes on automated plate readers or imaging systems
- Packaged in BacMam for robust mammalian cell expression
GENIe responding to 30μM SNP in HEK293 cells.
GENIe is a downward sensor, with bright initial green fluorescence that decreases in response to increased intracellular cGMP. As a single-color biosensor it can be combined with red fluorescent sensors to monitor simultaneous signals in the same cells or wells.
PDE Selectivity Assays
- Examine cAMP and cGMP responses to PDE inhibitors
- Examine PDE selectivity
- More information can be found in PDE Biology
Simple protocol:
Provided online is a simple cGMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.
- Text and video protocol online
- No lysis or complex liquid handling
- Single channel, non-FRET signal
Recent Publications
- JJ Bakhos, et al. Inhibition of Atrial Natriuretic Peptide Clearance Reduces Myocardial Fibrosis and Improves Cardiac Function in Diabetic Rats. bioRxiv. August 2024.
- RJ Lee, et al. Effects of Akt Activator SC79 on Human M0 Macrophage Phagocytosis and Cytokine Production. Cells. May 2024.
- R. Carey, et al. HSP90 Function is Required for T2R Bitter Taste Receptor Nitric Oxide Production and Innate Immune Responses in Human Airway Epithelial Cells and Macrophages. Cells. April 2022. (bioRxiv)
- I. Gapallawa, et al. Bitter taste receptors stimulate phagocytosis in human macrophages through calcium, nitric oxide, and cyclic-GMP signaling Cell. Mol. Life Sci. Feb, 2020 (bioRxiv)
GENIe – cGMP Assay Posters
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
Order a GENIe cGMP Assay Kit:
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#D0300R Red Down DAG Assay Kit
$895.00 – $1,795.00 -
Green Cell Stress Assay Kit #U0900G
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#D0800G Green GENIe cGMP Assay Kit
$895.00 – $1,795.00 -
#D0201G Green Cilia-targeted cADDis cAMP Assay Kit
$995.00 -
#D0400G Green PIP2 Assay Kit
$895.00 – $2,995.00 -
#D0400R Red PIP2 Assay Kit
$895.00 -
#C0500N Cre Recombinase
$995.00 -
#U0600R Red GECO Ca2+ Assay Kit
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Green/Red Ratiometric Cell Stress Assay Kit #U0901G
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#Z0400N Succinate Receptor 1
$995.00 -
#D0211G Ratiometric Cilia-Targeted cADDis cAMP Assay Kit
$995.00 -
#U0300R Red Up DAG Assay Kit
$895.00 – $1,795.00
