GENIe:
Live Cell cGMP Assays
Detect cGMP signaling with GENIe
- GENIe is a genetically-encoded fluorescent cGMP assay
- Measure cGMP signaling in real time with a single sensor
- Study cGMP production or PDE inhibition
- Screen for PDE inhibitors
- Examine PDE Biology
- Monitor fluorescence changes on automated plate readers or imaging systems
- Packaged in BacMam for robust mammalian cell expression
GENIe responding to 30μM SNP in HEK293 cells.
GENIe is a downward sensor, with bright initial green fluorescence that decreases in response to increased intracellular cGMP. As a single-color biosensor it can be combined with red fluorescent sensors to monitor simultaneous signals in the same cells or wells.
PDE Selectivity Assays
- Examine cAMP and cGMP responses to PDE inhibitors
- Examine PDE selectivity
- More information can be found in PDE Biology
Simple protocol:
Provided online is a simple cGMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.
- Text and video protocol online
- No lysis or complex liquid handling
- Single channel, non-FRET signal
Recent Publications
- JJ Bakhos, et al. Inhibition of Atrial Natriuretic Peptide Clearance Reduces Myocardial Fibrosis and Improves Cardiac Function in Diabetic Rats. bioRxiv. August 2024.
- RJ Lee, et al. Effects of Akt Activator SC79 on Human M0 Macrophage Phagocytosis and Cytokine Production. Cells. May 2024.
- R. Carey, et al. HSP90 Function is Required for T2R Bitter Taste Receptor Nitric Oxide Production and Innate Immune Responses in Human Airway Epithelial Cells and Macrophages. Cells. April 2022. (bioRxiv)
- I. Gapallawa, et al. Bitter taste receptors stimulate phagocytosis in human macrophages through calcium, nitric oxide, and cyclic-GMP signaling Cell. Mol. Life Sci. Feb, 2020 (bioRxiv)
GENIe – cGMP Assay Posters
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
- GENIe is a bright sensor compatible with standard fluorescent plate readers that produces a robust assay.
- GENIe can be paired with R-cADDis to compare cGMP and cAMP concentrations in living cells on a fluorescence plate reader.
- GENIe can detect the action of PDE inhibitors
- A comparison of the GENIe and cADDis sensors reveals the specificity of PDE inhibitors.
