iPSC-Derived Cell Assays
Express genetic constructs, label organelles, assess drug toxicity and more in living iPSC-derived cells!
Expression in iPSCs
Montana Molecular’s BacMam vectors robustly express fluorescent biosensors and other useful research tools in iPSC-derived cell types. The improved Big Sky vector expresses especially well in these cell types, even in the absence of HDAC inhibitors which are commonly used to boost and maintain BacMam expression.
BrainXell Cortical Glutamatergic iPSC-derived neurons were effectively transduced at any time from 3 days post-seeding and on – with strong expression for at least two weeks.
Mitochondria-targeted mNeonGreen expressed in Glutamatergic Cortical iPSC-derived neurons from BrainXell without HDAC inhibitor addition.
Live Cell Painting
Express red and green fluorescent proteins localized to subcellular domains using Montana Molecular’s Live Cell Painting tools. Observe spatial and temporal changes in cell morphology and response to drug addition in living cells, in a single well. No fixation, cell lysis, or staining required, though these techniques are compatible with our tools.
Endosome-targeted mNeonGreen expressed in BrainXell iPSC-derived neurons
Tau-targeted mNeonGreen and Nucleus-targeted Montana Paintbrush co-expressed in BrainXell iPSC-derived neurons
Identify iPSC Maturity with Cell Type-specific promoters
A cell type-specific promoter will specifically drive gene expression in target subpopulations of cells in a mixed culture, allowing noninvasive characterization and monitoring of living target cells in real time. Montana Molecular developed a BacMam vector to express the mNeonGreen fluorescent protein driven by a Synapsin promoter (#P1001G), which should limit expression to neuronal cell types.
In the context of iPSC-derived cells, we imagine this could be used to identify fully differentiated or “mature” cells of a given cell type. We tested BrainXell Cortical Glutamatergic iPSC-derived neurons with Synapsin-driven mNeonGreen, transducing cells on day 0, 1, 4 post-seeding. Cells remained negative for fluorescence until ~14 days post seeding, when they produced green fluorescence indicating mature neurons.
Synapsin-promoted mNeonGreen expressed in BrainXell iPSC-derived neurons
Assess toxicity using iPSCs
Express the fluorescent IRE1-XBP1 biosensor to assess cell stress responses and toxicity in living cells. In the videos below, see an example experiment using iPSC-derived peripheral neurons and cardiomyocytes from nCardia to screen for cardiotoxicity and neurotoxicity from a panel of chemotherapeutic compounds.
