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#C1204N 3CLPro Q189E Expression Kit


Express 3CLpro with the Q189E mutation in mammalian cells including HEK293, A549, and many more. Use alongside other 3CLpro mutant proteases and our 3CLglow biosensor assays to test 3CLpro inhibitor efficacy against a panel of mutations in the protease.



3CLpro (Mpro) Q189E mutation Expression Kit

Q189E, located within 6 Å of the nirmatrelvir binding site, is a naturally occurring mutation and has a two-fold increase in enzymatic activity compared to wild type 3CLpro (Hu et al. 2023). In a biochemical assay, Q189E reported a 19-fold increase in IC50 to nirmatrelvir resistance (Sasi et al. 2022). Additionally, a FRET-based enzymatic assay revealed a 10.7-fold increase in IC50 of nirmatrelvir (Huang et al. 2023).

  • Identify protease inhibitors that maintain efficacy against the Q189E mutation
  • Easy co-expression with our 3CLglow and 3CLglowUp sensors
  • Cost effective screening with minimal safety requirements (BSL-1)

Materials in the Kit:

• 3CLpro Q189E in BacMam
• Sodium Butyrate, 500 mM in H2O


Hu, Y., Lewandowski, E. M., Tan, H., Zhang, X., Morgan, R. T., Zhang, X., Jacobs, L. M., Butler, S. G., Gongora, M. V., Choy, J., Deng, X., Chen, Y., & Wang, J. (2023). Naturally occurring mutations of SARS-COV-2 main protease confer drug resistance to Nirmatrelvir. ACS Central Science, 9(8), 1658–1669.

Huang, C., Shuai, H., Qiao, J., Hou, Y., Zeng, R., Xia, A., Xie, L., Fang, Z., Li, Y., Yoon, C., Huang, Q., Hu, B., You, J., Quan, B., Zhao, X., Guo, N., Zhang, S., Ma, R., Zhang, J., … Yang, S. (2023). A new generation mpro inhibitor with potent activity against SARS-COV-2 omicron variants. Signal Transduction and Targeted Therapy, 8(1).

Sasi, V. M., Ullrich, S., Ton, J., Fry, S. E., Johansen-Leete, J., Payne, R. J., Nitsche, C., & Jackson, C. J. (2022). Predicting antiviral resistance mutations in SARS-COV-2 main protease with computational and experimental screening. Biochemistry, 61(22), 2495–2505.

Additional information


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

Protocol for Use


Kit Volume

5mL, 30mL

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