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3CLglow

A live cell assay for SARS-CoV-2 3CL protease
Order 3CLglow Assay Kit

3CLglow is a fluorescent biosensor for 3CLpro activity

  • 3C-like protease (3CLpro, Mpro, or Main protease) is essential for SARS-CoV-2 replication.
  • Discover new 3CL protease inhibitors.
  • BSL-1 kit is safe and easy to use.

Simple Assay Steps

  • Day 1 – Plate 39,000 HEK293 cells in each well.
  • Day 2 – Make transduction mix by combining BacMam, compounds, and sodium butyrate (included in 3CLglow Assay Kit).
  • Day 3 – Wash cells with PBS to remove auto fluorescent media and read fluorescence on a plate reader or imaging system.

Images captured with a BioTek LionHeart FX automated microscope

3CLglow – Measure 3CLpro Inhibition

  • GC376 inhibits 3CLpro enzyme and is a reliable control for 3CLglow assay.
  • EC50 value for GC376 is consistent with reports by Vuong et.al. 2020 using live SARS-CoV-2 in culture.
  • Compatible with detection on fluorescence plate readers or imaging systems.

GC376 inhibits 3CLpro and is a reliable control for 3CLglow assays.

HEK 293 cells expressing both SARS-CoV-2 3CLprotease and fluorescent biosensor. Images captured on the BioTek LionHeart imaging plate reader.