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#C1207N 3CLPro T21I + T304I Expression Kit

$395.00$795.00

Express 3CLpro with the T21I + T304I mutation in mammalian cells including HEK293, A549, and many more. Use alongside other 3CLpro mutant proteases and our 3CLglow biosensor assays to test 3CLpro inhibitor efficacy against a panel of mutations in the protease.


 

 

3CLpro (Mpro) T21I + T304I mutation Expression Kit

The T21I T304I variant emerged during escape studies under nirmatrelvir inhibition (Zhou et al. 2022). Using a short-term concentration response treatment in VeroE6 cells against the escape mutant T21I T304I, researchers observed a 5.9-fold shift in EC50 resistance to nirmatrelvir (Zhou et al. 2022). Pfizer reported a 3 to 8-fold increase in EC50 to nirmatrelvir using a biochemical assay (Pfizer Laboratories 2022).  

  • Identify protease inhibitors that maintain efficacy against the T21I + T304I mutation
  • Easy co-expression with our 3CLglow and 3CLglowUp sensors
  • Cost effective screening with minimal safety requirements (BSL-1)

Materials in the Kit:

• 3CLpro T21I + T304I in BacMam
• Sodium Butyrate, 500 mM in H2O

References:

Pfizer Laboratories. (2022). (rep.). FACT SHEET FOR HEALTHCARE PROVIDERS:  EMERGENCY USE AUTHORIZATION FOR PAXLOVID. Retrieved from https://labeling.pfizer.com/ShowLabeling.aspx?id=16474.

Zhou, Y., Gammeltoft, K. A., Ryberg, L. A., Pham, L. V., Tjørnelund, H. D., Binderup, A., Duarte Hernandez, C. R., Fernandez-Antunez, C., Offersgaard, A., Fahnøe, U., Peters, G. H., Ramirez, S., Bukh, J., & Gottwein, J. M. (2022). Nirmatrelvir-resistant SARS-COV-2 variants with high fitness in an infectious cell culture system. Science Advances, 8(51). https://doi.org/10.1126/sciadv.add7197

Additional information

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

Protocol for Use

https://montanamolecular.com/general-bacmam-transduction-protocol/

MSDS

https://montanamolecular.com/msds-for-sars-cov-2-reagents/

Kit Volume

5mL, 30mL

More Info

https://montanamolecular.com/detecting-inhibitors-of-mutant-3cl-proteases

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