#P1090G Omegasome targeted mNeon Green (Green OmegaPaint)
$595.00
mNeon Green fluorescent protein targeted to the Omegasome with ZNFN2A1. Packaged in Montana Molecular’s Big Sky BacMam vector for consistent, titratable transduction into a wide range of cell types.
Green OmegaPaint is a probe that combines the bright mNeonGreen fluorescent protein with a portion of the DFCP1 protein (Double FYVE Containing Protein One) (Gillooly et al., 2001). DFCP1 defines the omegasome, and contains two domains that specifically interact with the phosphatidylinositol 3-phosphate (PIP3) that is produced by the PI3-kinase VPS34 (Nähse et al., 2024). This unique kinase regulates autophagy, and without its activity phagsome formation is blocked. The omegasome membranes and structures are abundant and quite mobile in living cells, and the omegasome paint makes it possible to watch proteostasis in real time. However these are small structures that are best resolved using high numerical aperture objectives and confocal optics that reject out of focus light.
Materials in the Kit:
• Green OmegaPaint BacMam
• Sodium Butyrate, 500 mM in H2O
Additional information
| Protocol for Use | https://montanamolecular.com/live-cell-camp-assay-caddis/mneon-green-bacmam-protocol/ |
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| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
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| Kit Volume | 5mL |
| Terms of Sale |
