GENIe:
Live Cell cGMP Assays
Detect cGMP signaling with GENIe
- GENIe is a genetically-encoded fluorescent cGMP assay
- Measure cGMP signaling in real time with a single sensor
- Study cGMP production or PDE inhibition
- Screen for PDE inhibitors
- Examine PDE Biology
- Monitor fluorescence changes on automated plate readers or imaging systems
- Packaged in BacMam for robust mammalian cell expression
GENIe responding to 30μM SNP in HEK293 cells.
GENIe is a downward sensor, with bright initial green fluorescence that decreases in response to increased intracellular cGMP. As a single-color biosensor it can be combined with red fluorescent sensors to monitor simultaneous signals in the same cells or wells.
PDE Selectivity Assays
- Examine cAMP and cGMP responses to PDE inhibitors
- Examine PDE selectivity
- More information can be found in PDE Biology
Simple protocol:
Provided online is a simple cGMP assay protocol, with no need for co-factors, cell lysis, or complex liquid handling.
- Text and video protocol online
- No lysis or complex liquid handling
- Single channel, non-FRET signal
Recent Publications
- R. Carey, et al. HSP90 Function is Required for T2R Bitter Taste Receptor Nitric Oxide Production and Innate Immune Responses in Human Airway Epithelial Cells and Macrophages. Cells. April 2022. (bioRxiv)
- D. McMahon, et al. The bitter end: T2R bitter receptor agonists elevate nuclear calcium and induce apoptosis in non-ciliated airway epithelial cells. Cell Calcium. January 2022. (bioRxiv)
- I. Gapallawa, et al. Bitter taste receptors stimulate phagocytosis in human macrophages through calcium, nitric oxide, and cyclic-GMP signaling Cell. Mol. Life Sci. Feb, 2020 (bioRxiv)
GENIe – cGMP Assay Posters
PDE Specificity Illuminated: Monitoring cGMP and cAMP Levels in Living Cells
