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#D0231G Green Membrane-Targeted FMP cADDis cAMP

$795.00$1,695.00

Flag tagged MyrPalm cADDis cAMP sensor targeted to the plasma membrane. Genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types


Featured Publication:

Antidepressants produce persistent Gαs associated signaling changes in lipid rafts following drug withdrawal

Flag tagged MyrPalm cADDis cAMP sensor targeted to the plasma membrane. Monitor changes in cAMP in real time with fluorescence imaging. Genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines.


Materials in the Kit:

• Green Membrane-Targeted FMP cADDis cAMP

• Sodium Butyrate, 500 mM in H2O

β2 Adrenergic Receptor BacMam with nuclear red label

• Isoproterenol 10 mM, in 10mM HCl

To target the lipid rafts, the MyrPalm sequence from Lyn Kinase is fused to the cAADis sensor.  This has been used in several studies to follow cAMP signaling at the lipid (Agarwal et al., 2017, 2018)

Additional information

Protocol for Use

https://montanamolecular.com/targeted-caddis-camp-assay-protocol/

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

MSDS

https://montanamolecular.com/msds-for-caddis-assays/

More Info

https://montanamolecular.com/live-cell-camp-assay-caddis

Scientific Publications

https://montanamolecular.com/scientific-publications

Kit Volume

10mL, 30mL

GPCR Assay References
cADDis cAMP Assay References