#U0263G AKAP Targeted cADDis cAMP Sensor – AKAP1

U0263G is a fusion of the complete AKAP1 protein to the cADDis biosensor. In HEK293 cells, the AKAP1-targeted cADDis sensor localizes to the mitochondria.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP1-targeted cADDis cAMP sensor. Increase in fluorescence indicates increase in cAMP presence. cADDis is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.

Materials in the Kit:

• Green AKAP1-targeted cADDis cAMP sensor BacMam
• Sodium Butyrate, 500 mM in H₂O
• β2 Adrenergic Receptor BacMam
• Isoproterenol 10 mM, in 10mM HCl

AKAP1

The gene AKAP1 (also known as AKAP149) encodes several different splice variants that localize differently in the cell.  One of these targets the cADDis cAMP sensor to the mitochondrial outer membrane.  One of these targets the cADDis cAMP sensor to the mitochondrial outer membrane (Huang et al., 1999).  AKAP1 is a dual AKAP, with the ability to bind both regulatory subunits of PKA. 

AKAP1 plays several intracellular roles.  At the mitochondria, AKAP1 is thought to organize PKA activity and phosphorylation of crucial mitochondrial proteins, including Drp1 (Dickey & Strack, 2011).  There is a variety of evidence that AKAP1 is regulating fundamental mitochondrial biology (Zaccolo et al., 2021). In the cardiac system, AKAP1 is also thought to play a role in the cardiac stress response (Ercu & Klussmann, 2018). AKAP1 also appears to play a role in the protection of neurons (Dagda et al., 2011; Merrill et al., 2011) as well as in morphogenesis (Dickey & Strack, 2011).